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Literature summary for 1.2.7.8 extracted from
- In vitro heat effect on heterooligomeric subunit assembly of thermostable indolepyruvate ferredoxin oxidoreductase (1998), FEBS Lett., 434, 372-376.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cloning and sequence analysis of the IOR gene, 2 genes iorA and iorB, encoding alpha and beta subunits are tandemly arranged, KOD1-IOR overproduced in anaerobically incubated Escherichia coli BL21(DE3) | Thermococcus kodakarensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
| 71000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
| 192000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Pyrococcus furiosus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r |  |
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Thermococcus kodakarensis | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
| Thermococcus kodakarensis | - |
KOD1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus kodakarensis | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| indolepyruvate + CoA + oxidized methylviologen | - |
Pyrococcus furiosus | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
| indolepyruvate + CoA + oxidized methylviologen | - |
Thermococcus kodakarensis | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
| p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus kodakarensis | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus kodakarensis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Thermococcus kodakarensis |
| tetramer | 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermococcus kodakarensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 80 | - |
Thermococcus kodakarensis |
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Release 2023.1 (January 2023)
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