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Literature summary for 1.2.7.8 extracted from
- Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum (1997), Eur. J. Biochem., 244, 862-868.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
CoA | pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus |  |
| 1.4 | - |
phenylpyruvate | pH 8.0, 65°C | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 67000 | - |
2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Methanothermobacter thermautotrophicus | biosynthesis of amino acids from fatty acids | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.15 | - |
- |
Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | biosynthesis of amino acids from fatty acids | Methanothermobacter thermautotrophicus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| phenylpyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 67000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Methanothermobacter thermautotrophicus |
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