Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.7.5 extracted from

  • Roy, R.; Mukund, S.; Schut, G.J.; Dunn, D.M.; Weiss, R.; Adams, M.W.
    Purification and molecular characterization of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus: the third of a putative five-member tungstoenzyme family (1999), J. Bacteriol., 181, 1171-1180.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Sulfide activation of formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

General Stability

General Stability Organism
10% glycerol and 2 mM DTT stabilize during purification Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information formaldehyde ferredoxin oxidoreductase, Km for several substrates at different concentrations with purified and sulfide-activated enzyme, overview Pyrococcus furiosus
25
-
formaldehyde formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.4 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
Fe2+ 3.8 gatoms per subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
Mg2+ 1.5 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
Tungsten 0.9 gatom per subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE Pyrococcus furiosus
275000
-
formaldehyde ferredoxin oxidoreductase, gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus
-
an acid + H+ + reduced ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
hyperthermophilic archaeon
-
Pyrococcus furiosus
-
strain DSM 3638
-
Pyrococcus furiosus
-
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase
-
Pyrococcus furiosus
-
genes aor and for
-

Purification (Commentary)

Purification (Comment) Organism
formaldehyde ferredoxin oxidoreductase, 17fold Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
formaldehyde ferredoxin oxidoreductase, substrate specificity Pyrococcus furiosus
42
-
purified formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus acetate + H+ + reduced benzyl viologen
-
ir
acetaldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus acetate + H+ + reduced ferredoxin
-
ir
an aldehyde + H2O + oxidized ferredoxin
-
Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
?
an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
ir
butyraldehyde + H2O + oxidized ferredoxin low activity Pyrococcus furiosus butyrate + H+ + reduced ferredoxin
-
ir
butyraldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus butyrate + H+ + reduced ferredoxin
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen low activity, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus crotonate + H+ + reduced benzyl viologen
-
ir
formaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
ir
formaldehyde + H2O + oxidized ferredoxin formaldehyde and aldehyde ferredoxin oxidoreductases Pyrococcus furiosus formate + H+ + reduced ferredoxin
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase, no activity Pyrococcus furiosus isovalerate + H+ + reduced benzyl viologen
-
?
propionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus propionate + H+ + reduced benzyl viologen
-
ir
propionaldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus propionate + H+ + reduced ferredoxin
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
FOR
-
Pyrococcus furiosus
More formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 formaldehyde ferredoxin oxidoreductase, activity increases 4.5fold from 60°C to 90°C at pH 8.4 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme is thermostable Pyrococcus furiosus
80
-
formaldehyde ferredoxin oxidoreductase, t1/2: 8 h, in presence of 2 mM dithionite and 2 mM DTT, pH 8.4 Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
assay at Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 formaldehyde ferredoxin oxidoreductase, activity increases linearly from pH 5.5 to pH 10.0 at 80°C Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
Pyrococcus furiosus
form A cofactor formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus