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Literature summary for 1.2.7.5 extracted from

  • Heider, J.; Ma, K.; Adams, M.W.W.
    Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1 (1995), J. Bacteriol., 177, 4757-4764.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.7.5

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl competitive to methyl viologen Thermococcus sp.
acetaldehyde 50% inhibition at 5 mM Thermococcus sp.
aldehydes substrate inhibition at high concentrations, overview Thermococcus sp.
arsenite 50% inhibition at 0.5 mM Thermococcus sp.
crotonaldehyde 50% inhibition at 20 mM Thermococcus sp.
iodoacetate 50% inhibition at 0.5 mM Thermococcus sp.
isovalerylaldehyde 50% inhibition at 4 mM Thermococcus sp.
p-chloromercuribenzoate
-
 Thermococcus sp.
phenylacetaldehyde 50% inhibition at 7 mM Thermococcus sp.
propionaldehyde 50% inhibition at 0.6 mM Thermococcus sp.
Zn2+ 50% inhibition at 0.25 mM Thermococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 oxidized ferredoxin ES-1 ferredoxin Thermococcus sp.
0.01
-
 oxidized ferredoxin pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
0.016
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.028
-
 isovalerylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.05
-
 Indoleacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.057
-
 benzaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.065
-
 Salicylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.076
-
 phenylacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.13
-
 crotonaldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
0.14
-
 crotonaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.15
-
 propionaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.17
-
 benzyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
0.2
-
 glyceraldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
1.42
-
 formaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.8
-
 acetate pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
2.9
-
 methyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.7 gatoms per subunit Thermococcus sp.
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Thermococcus sp.
Mg2+ contains 1 Mg-atom per subunit Thermococcus sp.
Molybdenum
-
 Thermococcus sp.
Tungsten 1 tungsten per subunit Thermococcus sp.
Zn2+ 0.2 gatoms per subunit Thermococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 2 * 67000, SDS-PAGE Thermococcus sp.
105000
-
 gel filtration Thermococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin Thermococcus sp. enzyme oxidizes aldehydes generated during amino acid catabolism an acid + H+ + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
 hyperthermophilic archaeon
-
Thermococcus sp. ES1
-
 hyperthermophilic archaeon
-

Oxidation Stability

Oxidation Stability Organism
very sensitive to O2, after 1 h at 60°C 3% activity remains, after 1 h at 4°C 39% activity remains Thermococcus sp.

Purification (Commentary)

Purification (Comment) Organism
about 120fold, to homogeneity Thermococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
238
-
 purified enzyme Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. acetate + H+ + reduced benzyl viologen
-
 ir
acetaldehyde + H2O + oxidized ferredoxin best substrate Thermococcus sp. acetate + H+ + reduced ferredoxin
-
 ir
acetate + H+ + reduced methyl viologen very low activity Thermococcus sp. acetaldehyde + H2O + oxidized methyl viologen
-
 ir
acetate + H+ + reduced methyl viologen below pH 6.0 Thermococcus sp. acetaldehyde + H2O + oxidized methyl viologen
-
 ir
an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ir
an aldehyde + H2O + oxidized ferredoxin enzyme oxidizes aldehydes generated during amino acid catabolism Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ?
benzaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. benzoic acid + H+ + reduced benzyl viologen
-
 ir
crotonaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. crotonate + H+ + reduced benzyl viologen
-
 ir
crotonaldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. crotonate + H+ + reduced ferredoxin
-
 r
formaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. formate + H+ + reduced benzyl viologen
-
 ir
glyceraldehyde + H2O + oxidized methyl viologen very poor substrate Thermococcus sp. glycerate + H+ + reduced methyl viologen
-
 ?
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. indoleacetate + H+ + reduced benzyl viologen
-
 ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. isovalerate + H+ + reduced benzyl viologen
-
 ir
additional information acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate Thermococcus sp. ?
-
 ?
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. phenylacetate + H+ + reduced benzyl viologen
-
 ir
propionaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. propionate + H+ + reduced benzyl viologen
-
 ir
salicylaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. salicylic acid + H+ + reduced benzyl viologen
-
 ir

Subunits

Subunits Comment Organism
dimer 2 * 67000, SDS-PAGE Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme is thermostable Thermococcus sp.
23
-
 pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min Thermococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
 acetate pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
2.8
-
 glyceraldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
13
-
 Salicylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
22
-
 crotonaldehyde pH 8.4, 45°C, benzyl viologen as electron acceptor Thermococcus sp.
55
-
 Indoleacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
62
-
 methyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
250
-
 benzyl viologen pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
269
-
 crotonaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
270
-
 oxidized ferredoxin ES-1 ferredoxin, pH 8.4, 85°C, with crotonaldehyde Thermococcus sp.
272
-
 isovalerylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
343
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
720
-
 benzaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
950
-
 formaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
960
-
 phenylacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1100
-
 propionaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 assay at Thermococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 oxidation of acetaldehyde with benzyl viologen as electron acceptor Thermococcus sp.

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Thermococcus sp.
form A cofactor in presence of iodine Thermococcus sp.
form B cofactor in absence of iodine Thermococcus sp.
molybdopterin
-
 Thermococcus sp.