Literature summary for 1.2.7.4 extracted from

Kim, E.J.; Feng, J.; Bramlett, M.R.; Lindahl, P.A.
Evidence for a proton transfer network and a required persulfide-bond-forming cysteine residue in Ni-containing carbon monoxide dehydrogenases (2004), Biochemistry, 43, 5728-5734.

Search for more literature for 1.2.7.4

Protein Variants

Protein Variants	Comment	Organism
A121H/H122A	11% of wild type activity	Moorella thermoacetica
C316S	0.3% of wild type activity	Moorella thermoacetica
E115H/H116A	24% of wild type activity	Moorella thermoacetica
H113A	44% of wild type activity, in presence of imidazole, 45% of wild type activity	Moorella thermoacetica
H113A/H116A/H119A	no activity	Moorella thermoacetica
H113A/H119A	15% of wild type activity	Moorella thermoacetica
H116A	6% of wild type activity, in presence of imidazole, 3% of wild type activity	Moorella thermoacetica
H116A/G117H	0.8% of wild type activity	Moorella thermoacetica
H116C	46% of wild type activity	Moorella thermoacetica
H116D	0.4% of wild type activity	Moorella thermoacetica
H119A	27% of wild type activity	Moorella thermoacetica
H122A	3% of wild type activity	Moorella thermoacetica
H122A/A123H	72% of wild type activity	Moorella thermoacetica
K587A	42% of wild type activity	Moorella thermoacetica
K587A/H113A	0.7% of wild type activity	Moorella thermoacetica
N284A	41% of wild type activity	Moorella thermoacetica
N284A/H119A	36% of wild type activity	Moorella thermoacetica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	-	Moorella thermoacetica
Nickel	-	Moorella thermoacetica

Organism

Organism	UniProt	Comment	Textmining
Moorella thermoacetica	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO + H2O + acceptor	-	Moorella thermoacetica	CO2 + reduced acceptor	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Moorella thermoacetica

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	-	about 60% of activity at pH 9.0	Moorella thermoacetica

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Release 2023.1 (January 2023)