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Literature summary for 1.2.7.4 extracted from

  • Ramer, S.E.; Raybuck, S.A.; Orme-Johnson, W.H.; Walsh, C.T.
    Kinetic characterization of the [3'-32P]coenzyme A/acetyl coenzyme A exchange catalyzed ba a three-subunit form of the carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum (1989), Biochemistry, 28, 4675-4680.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CoASH
-
Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
CoA acetyl-CoA/CoA exchange reaction Moorella thermoacetica
1.5
-
acetyl-CoA acetyl-CoA/CoA exchange reaction Moorella thermoacetica
5
-
propionyl-CoA
-
Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000 + 2 * 71000 + 2 * 78000, alpha2beta2gamma2, SDS-PAGE Moorella thermoacetica
71000
-
2 * 50000 + 2 * 71000 + 2 * 78000, alpha2beta2gamma2, SDS-PAGE Moorella thermoacetica
78000
-
2 * 50000 + 2 * 71000 + 2 * 78000, alpha2beta2gamma2, SDS-PAGE Moorella thermoacetica
410000
-
gel filtration Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-

Reaction

Reaction Comment Organism Reaction ID
CO + H2O + 2 oxidized ferredoxin = CO2 + 2 reduced ferredoxin + 2 H+ enzyme also catalyzes acetyl-CoA/CoA and acetyl-CoA/CO exchange reaction, enzyme has both CO-dehydrogenase and acetyl-CoA synthase activity Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-SCoA + CO acetyl-CoA/CO exchange reaction, 14C experiments prove that the enzyme can cleave both the carbon-carbon and carbon-sulfur bonds of acetyl-CoA as well as to store methyl, CO, and CoA fragments at the active site Moorella thermoacetica acetyl-SCoA + CO
-
r
acetyl-SCoA + CoASH acetyl-CoA/CoA exchange reaction Moorella thermoacetica acetyl-SCoA + CoASH
-
r
CO + H2O + methyl viologen catalyzes the exchange reaction between coenzyme A and acetyl-CoA Moorella thermoacetica CO2 + reduced methyl viologen
-
?
CO + H2O + methyl viologen catalyzes the exchange reaction between propionyl-CoA and CoA at 7% of the exchange with acetyl-CoA Moorella thermoacetica CO2 + reduced methyl viologen
-
?
propionyl-SCoA + CoASH acetyl-CoA/CoA exchange reaction Moorella thermoacetica propionyl-SCoA + CoASH
-
r

Subunits

Subunits Comment Organism
hexamer 2 * 50000 + 2 * 71000 + 2 * 78000, alpha2beta2gamma2, SDS-PAGE Moorella thermoacetica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
CoASH
-
Moorella thermoacetica