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Literature summary for 1.2.7.12 extracted from
- Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum. Identification and characterization of a tungsten isoenzyme of formylmethanofuran dehydrogenase (1994), Arch. Microbiol., 161, 220-228.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
N-formylmethanofuran | - |
Methanothermobacter thermautotrophicus |  |
| 0.8 | - |
methylviologen | - |
Methanothermobacter thermautotrophicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 8 mol of non-heme iron and 8 mol of acid-labile sulfur per mol of enzyme | Methanothermobacter thermautotrophicus | |
| Tungsten | tungsten-containing isoenzyme: contains 0.4 mol of tungsten per mol of enzyme | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 31000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 53000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 65000 | - |
1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 160000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| tungsten-containing isoenzyme | Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter thermautotrophicus | methanofuran + reduced methylviologen + CO2 | - |
? | |
| formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methanofuran + reduced methylviologen + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Methanothermobacter thermautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pterin | contains molybdopterin guanine dinucleotide as pterin cofactor | Methanothermobacter thermautotrophicus | |
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Release 2023.1 (January 2023)
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