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Literature summary for 1.2.7.12 extracted from
- Molybdopterin adenine dinucleotide and molybdopterin hypoxanthine dinucleotide in formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum (Marburg) (1991), FEBS Lett., 290, 31-34.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
N-formylmethanofuran | - |
Methanothermobacter thermautotrophicus |  |
| 0.1 | - |
methanofuran | methylviologen | Methanothermobacter thermautotrophicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 4 mol of non-heme iron and 4 mol of acid-labile sulfur per mol of enzyme | Methanothermobacter thermautotrophicus | |
| Molybdenum | contains 0.3-0.6 mol of molybdenum per mol of enzyme | Methanothermobacter thermautotrophicus | |
| Tungsten | enzyme exists as a molybdenum-containing isoenzyme and a tungsten-containing isoenzyme | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen | - |
Methanothermobacter thermautotrophicus | CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen | - |
? | |
| formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen | - |
? | |
| formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter thermautotrophicus | methanofuran + reduced methylviologen + CO2 | - |
? | |
| formylmethanofuran + oxidized methylviologen + H2O | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methanofuran + reduced methylviologen + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 3 different polypeptides are detected by SDS-PAGE: MW 60000, MW 45000 and MW 38000 | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Methanothermobacter thermautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pterin | contains 0.5-0.7 mol of a pterin cofactor of MW 800000 per mol of enzyme, contains di(carboxamidomethyl)-derivatives of molybdopterin guanine dinucleotide, of molybdopterin adenine dinucleotide and of molybdopterin hypoxanthine dinucleotide in the ratio 1.0:1.0:0.1 | Methanothermobacter thermautotrophicus | |
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