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Literature summary for 1.2.7.12 extracted from

  • Vorholt, J.A.; Vaupel, M.; Thauer, R.K.
    A polyferredoxin with eight [4Fe-4S] clusters as a subunit of molybdenum formylmethanofuran dehydrogenase from Methanosarcina barkeri (1996), Eur. J. Biochem., 236, 309-317.
    View publication on PubMed
Search for more literature for 1.2.7.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of the six genes of the fmd operon in Escherichia coli Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 N-Furfurylformamide molybdenum enzyme Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains approximately 30 mol non-heme iron per mol and 30 mol acid-labile sulfur per mol. Subunit F contains 4 tandemly repeated bacterial-ferredoxin-like domains and is predicted to be a polyferredoxin that contains 32 iron atoms in eight [4Fe-4S] clusters Methanosarcina barkeri
Molybdenum enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 6 different types of subunits, x * 65100, subunit FmdA, + x * 31600, subunit FmdC, + x * 22800, subunit FmdE, + x * 14000, subunit FmdD, + x * ? + x * ?, calculation from nucleotide sequence Methanosarcina barkeri
22800
-
 6 different types of subunits, x * 65100, subunit FmdA, + x * 31600, subunit FmdC, + x * 22800, subunit FmdE, + x * 14000, subunit FmdD, + x * ? + x * ?, calculation from nucleotide sequence Methanosarcina barkeri
31600
-
 6 different types of subunits, x * 65100, subunit FmdA, + x * 31600, subunit FmdC, + x * 22800, subunit FmdE, + x * 14000, subunit FmdD, + x * ? + x * ?, calculation from nucleotide sequence Methanosarcina barkeri
65100
-
 6 different types of subunits, x * 65100, subunit FmdA, + x * 31600, subunit FmdC, + x * 22800, subunit FmdE, + x * 14000, subunit FmdD, + x * ? + x * ?, calculation from nucleotide sequence Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methanosarcina barkeri enzyme is involved in methanogenesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is involved in methanogenesis Methanosarcina barkeri ?
-
 ?
N-Furfurylformamide + oxidized acceptor
-
 Methanosarcina barkeri ?
-
 ?

Subunits

Subunits Comment Organism
? 6 different types of subunits, x * 65100, subunit FmdA, + x * 31600, subunit FmdC, + x * 22800, subunit FmdE, + x * 14000, subunit FmdD, + x * ? + x * ?, calculation from nucleotide sequence Methanosarcina barkeri