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Literature summary for 1.2.7.11 extracted from

  • Iwasaki, T.; Oshima, T.
    Ferredoxin and related enzymes from Sulfolobus (2001), Methods Enzymol., 334, 3-22.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
pyruvate pH and temperature not specified in the publication Sulfolobus sp.
0.87
-
2-oxoglutarate pH and temperature not specified in the publication Sulfolobus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
iron-sulfur centre the enzyme contains one [4Fe-4S]2+,1+ cluster Sulfolobus sp.
Mg2+ contains two Mg2+ per dimer Sulfolobus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
1 * 70000 (alpha) + 1 * 37000 (beta) Sulfolobus sp.
70000
-
1 * 70000 (alpha) + 1 * 37000 (beta) Sulfolobus sp.

Organism

Organism UniProt Comment Textmining
Sulfolobus sp. P72578 and P72579 P72578: alpha-subunit, and P72579: beta-subunit
-
Sulfolobus sp. 7 P72578 and P72579 P72578: alpha-subunit, and P72579: beta-subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + CoA + oxidized ferredoxin
-
Sulfolobus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxobutyrate + CoA + oxidized ferredoxin
-
Sulfolobus sp. 7 propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
Sulfolobus sp. succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
Sulfolobus sp. 7 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
pyruvate + CoA + 2 oxidized ferredoxin
-
Sulfolobus sp. acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
pyruvate + CoA + 2 oxidized ferredoxin
-
Sulfolobus sp. 7 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 70000 (alpha) + 1 * 37000 (beta) Sulfolobus sp.

Synonyms

Synonyms Comment Organism
2-oxoacid:ferredoxin oxidoreductase
-
Sulfolobus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Sulfolobus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Sulfolobus sp.

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre the enzyme contains one [4Fe-4S]2+,1+ cluster Sulfolobus sp.
thiamine diphosphate enzyme contains one thiamine diphosphate Sulfolobus sp.