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Literature summary for 1.2.7.11 extracted from

  • Park, Y.J.; Yoo, C.B.; Choi, S.Y.; Lee, H.B.
    Purifications and characterizations of a ferredoxin and its related 2-oxoacid:ferredoxin oxidoreductase from the hyperthermophilic archaeon, Sulfolobus solfataricus P1 (2006), J. Biochem. Mol. Biol., 39, 46-54.
    View publication on PubMed
Search for more literature for 1.2.7.11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.163
-
 2-oxoglutarate pH 7.0, 55°C Saccharolobus solfataricus
0.275
-
 pyruvate pH 7.0, 55°C Saccharolobus solfataricus
0.516
-
 2-oxobutyrate pH 7.0, 55°C Saccharolobus solfataricus
0.516
-
 2-oxobutanoate pH 7.0, 55°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 1 * 69000 (alpha-subunit) + 1 * 34000 (beta-subunit), SDS-PAGE, MALDI-TOF mass spectrometry Saccharolobus solfataricus
34000
-
 1 * 69000, alpha-subuit, + 1 * 34000, beta-subunit, SDS-PAGE and MALDI-TOF Saccharolobus solfataricus
69000
-
 1 * 69000 (alpha-subunit) + 1 * 34000 (beta-subunit), SDS-PAGE, MALDI-TOF mass spectrometry Saccharolobus solfataricus
69000
-
 1 * 69000, alpha-subuit, + 1 * 34000, beta-subunit, SDS-PAGE and MALDI-TOF Saccharolobus solfataricus
103000
-
 native PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus DSM 1616
-
-
-
Saccharolobus solfataricus P1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.6
-
 pH 7.0, 55°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + CoA + 2 oxidized cytochrome c
-
 Saccharolobus solfataricus propanoyl-CoA + CO2 + 2 reduced cytochrome c + 2 H+
-
 ?
2-oxobutyrate + CoA + oxidized ferredoxin Sulfolobus solfataricus ferredoxin contains a dicluster, i.e., a [3Fe-4S] cluster and a [4Fe-4S] cluster, and to possibly contain one zinc center Saccharolobus solfataricus propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-oxobutyrate + CoA + oxidized ferredoxin Sulfolobus solfataricus ferredoxin contains a dicluster, i.e., a [3Fe-4S] cluster and a [4Fe-4S] cluster, and to possibly contain one zinc center Saccharolobus solfataricus P1 propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-oxobutyrate + CoA + oxidized ferredoxin Sulfolobus solfataricus ferredoxin contains a dicluster, i.e., a [3Fe-4S] cluster and a [4Fe-4S] cluster, and to possibly contain one zinc center Saccharolobus solfataricus DSM 1616 propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-oxoglutarate + CoA + 2 oxidized cytochrome c best substrate tested Saccharolobus solfataricus succinyl-CoA + CO2 + 2 reduced cytochrome c + 2 H+
-
 ?
2-oxoglutarate + CoA + 2 oxidized ferredoxin relative activity is 20.4% compared to 2-oxoglutarate, kcat/Km for 2-oxoglutarate is 6.5% compared to kcat/Km for 2-oxobutyrate Saccharolobus solfataricus succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
2-oxoglutarate + CoA + 2 oxidized ferredoxin relative activity is 20.4% compared to 2-oxoglutarate, kcat/Km for 2-oxoglutarate is 6.5% compared to kcat/Km for 2-oxobutyrate Saccharolobus solfataricus P1 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
2-oxoglutarate + CoA + 2 oxidized ferredoxin relative activity is 20.4% compared to 2-oxoglutarate, kcat/Km for 2-oxoglutarate is 6.5% compared to kcat/Km for 2-oxobutyrate Saccharolobus solfataricus DSM 1616 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
additional information no activity with glyoxylate Saccharolobus solfataricus ?
-
 ?
additional information enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutanoate, and 2-oxoglutarate Saccharolobus solfataricus ?
-
 ?
additional information no activity with glyoxylate Saccharolobus solfataricus P1 ?
-
 ?
additional information enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutanoate, and 2-oxoglutarate Saccharolobus solfataricus P1 ?
-
 ?
additional information no activity with glyoxylate Saccharolobus solfataricus DSM 1616 ?
-
 ?
additional information enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutanoate, and 2-oxoglutarate Saccharolobus solfataricus DSM 1616 ?
-
 ?
pyruvate + CoA + 2 oxidized cytochrome c
-
 Saccharolobus solfataricus acetyl-CoA + CO2 + 2 reduced cytochrome c + 2 H+
-
 ?
pyruvate + CoA + 2 oxidized ferredoxin relative activity is 31.2% compared to 2-oxoglutarate, kcat/Km for pyruvate is 19% compared to kcat/Km for 2-oxobutyrate Saccharolobus solfataricus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
pyruvate + CoA + 2 oxidized ferredoxin relative activity is 31.2% compared to 2-oxoglutarate, kcat/Km for pyruvate is 19% compared to kcat/Km for 2-oxobutyrate Saccharolobus solfataricus P1 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
pyruvate + CoA + 2 oxidized ferredoxin relative activity is 31.2% compared to 2-oxoglutarate, kcat/Km for pyruvate is 19% compared to kcat/Km for 2-oxobutyrate Saccharolobus solfataricus DSM 1616 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 69000, alpha-subuit, + 1 * 34000, beta-subunit, SDS-PAGE and MALDI-TOF Saccharolobus solfataricus
heterodimer 1 * 69000 (alpha-subunit) + 1 * 34000 (beta-subunit), SDS-PAGE, MALDI-TOF mass spectrometry Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Saccharolobus solfataricus
70
-
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 2-oxobutanoate pH 7.0, 55°C Saccharolobus solfataricus
1.55
-
 2-oxobutyrate pH 7.0, 55°C Saccharolobus solfataricus
2.4
-
 pyruvate pH 7.0, 55°C Saccharolobus solfataricus
7.5
-
 2-oxoglutarate pH 7.0, 55°C Saccharolobus solfataricus
7.53
-
 2-oxoglutarate pH 7.0, 55°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Saccharolobus solfataricus
7
-
 assay at Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 50% of maximal activity, pH 9.0: about 50% of maximal activity Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin enzyme contains a dicluster, [3Fe-4S][4Fe-4S], type ferredoxin of 11000 Da by SDS-PAGE and of 11180 Da by MALDI-TOF mass spectrometry Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 2-oxobutyrate pH 7.0, 55°C Saccharolobus solfataricus
3
-
 2-oxobutanoate pH 7.0, 55°C Saccharolobus solfataricus
8.7
-
 pyruvate pH 7.0, 55°C Saccharolobus solfataricus
8.73
-
 pyruvate pH 7.0, 55°C Saccharolobus solfataricus
46
-
 2-oxoglutarate pH 7.0, 55°C Saccharolobus solfataricus
46.2
-
 2-oxoglutarate pH 7.0, 55°C Saccharolobus solfataricus