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Literature summary for 1.2.7.10 extracted from

  • Pierce, E.; Becker, D.F.; Ragsdale, S.W.
    Identification and characterization of oxalate oxidoreductase, a novel thiamine pyrophosphate-dependent 2-oxoacid oxidoreductase that enables anaerobic growth on oxalate (2010), J. Biol. Chem., 285, 40515-40524.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
oxalate in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C Moorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains 14 mol iron per mol protein Moorella thermoacetica
Mg2+ the enzyme contains 0.8 mol Mg2+ per mol protein Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica
33900
-
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
34200
-
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
36000
-
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica
43000
-
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica
43700
-
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
226900
-
analytical ultracentrifugation Moorella thermoacetica
236000
-
gel filtration Moorella thermoacetica
243000
-
native PAGE Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, red agarose column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography Moorella thermoacetica

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:glucose-grown cell
-
Moorella thermoacetica
-
culture condition:oxalate-grown cell
-
Moorella thermoacetica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
enzyme from glucose-grown cells, at pH 7.9 and 55°C Moorella thermoacetica
0.4
-
enzyme from oxalate-grown cells, at pH 7.9 and 55°C Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + methyl viologen
-
Moorella thermoacetica ?
-
?
2-oxoglutarate + H2O + methyl viologen
-
Moorella thermoacetica ?
-
?
2-oxovalerate + H2O + methyl viologen very low activity Moorella thermoacetica ?
-
?
glyoxylate + methyl viologen
-
Moorella thermoacetica ?
-
?
additional information OOR does not use formate, coenzyme A, ATP, NAD+, and NADP+ and as a substrate Moorella thermoacetica ?
-
?
oxalate + cytochrome c cytochrome c is the best electron acceptor Moorella thermoacetica CO2 + reduced cytochrome c
-
?
oxalate + FAD
-
Moorella thermoacetica CO2 + FADH2
-
?
oxalate + ferredoxin oxalate is the best substrate Moorella thermoacetica CO2 + reduced ferredoxin
-
?
oxalate + FMN
-
Moorella thermoacetica CO2 + FMNH2
-
?
oxalate + methyl viologen
-
Moorella thermoacetica CO2 + reduced methyl viologen
-
?
oxalate + metronidazole
-
Moorella thermoacetica CO2 + reduced metronidazole
-
?
oxaloacetate + H2O + methyl viologen
-
Moorella thermoacetica ?
-
?
pyruvate + methyl viologen
-
Moorella thermoacetica ?
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
heterotrimer 1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica

Synonyms

Synonyms Comment Organism
OOR
-
Moorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
oxalate in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center the enzyme contains three [Fe4S4] clusters Moorella thermoacetica
thiamine diphosphate dependent on Moorella thermoacetica