KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
oxalate | in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C | Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains 14 mol iron per mol protein | Moorella thermoacetica | |
Mg2+ | the enzyme contains 0.8 mol Mg2+ per mol protein | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE | Moorella thermoacetica |
33900 | - |
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence | Moorella thermoacetica |
34200 | - |
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence | Moorella thermoacetica |
36000 | - |
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE | Moorella thermoacetica |
43000 | - |
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE | Moorella thermoacetica |
43700 | - |
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence | Moorella thermoacetica |
226900 | - |
analytical ultracentrifugation | Moorella thermoacetica |
236000 | - |
gel filtration | Moorella thermoacetica |
243000 | - |
native PAGE | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, red agarose column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography | Moorella thermoacetica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:glucose-grown cell | - |
Moorella thermoacetica | - |
culture condition:oxalate-grown cell | - |
Moorella thermoacetica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
enzyme from glucose-grown cells, at pH 7.9 and 55°C | Moorella thermoacetica |
0.4 | - |
enzyme from oxalate-grown cells, at pH 7.9 and 55°C | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + methyl viologen | - |
Moorella thermoacetica | ? | - |
? | |
2-oxoglutarate + H2O + methyl viologen | - |
Moorella thermoacetica | ? | - |
? | |
2-oxovalerate + H2O + methyl viologen | very low activity | Moorella thermoacetica | ? | - |
? | |
glyoxylate + methyl viologen | - |
Moorella thermoacetica | ? | - |
? | |
additional information | OOR does not use formate, coenzyme A, ATP, NAD+, and NADP+ and as a substrate | Moorella thermoacetica | ? | - |
? | |
oxalate + cytochrome c | cytochrome c is the best electron acceptor | Moorella thermoacetica | CO2 + reduced cytochrome c | - |
? | |
oxalate + FAD | - |
Moorella thermoacetica | CO2 + FADH2 | - |
? | |
oxalate + ferredoxin | oxalate is the best substrate | Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
oxalate + FMN | - |
Moorella thermoacetica | CO2 + FMNH2 | - |
? | |
oxalate + methyl viologen | - |
Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
oxalate + metronidazole | - |
Moorella thermoacetica | CO2 + reduced metronidazole | - |
? | |
oxaloacetate + H2O + methyl viologen | - |
Moorella thermoacetica | ? | - |
? | |
pyruvate + methyl viologen | - |
Moorella thermoacetica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence | Moorella thermoacetica |
heterotrimer | 1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE | Moorella thermoacetica |
Synonyms | Comment | Organism |
---|---|---|
OOR | - |
Moorella thermoacetica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
oxalate | in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | the enzyme contains three [Fe4S4] clusters | Moorella thermoacetica | |
thiamine diphosphate | dependent on | Moorella thermoacetica |