Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | dithiothreitol has no influence on the activity | Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | the addition of 0.3 M NaCl causes a significant inhibition (up to 80%) of the activity | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
oxidized ferredoxin 2 | in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii | |
0.0046 | - |
oxidized ferredoxin 1 | in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii | |
0.0052 | - |
CoA | in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii | |
0.16 | - |
pyruvate | in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | upon addition of Mg2+, an ion that stabilizes thiamine diphosphate, the enzymatic activity almost doubles | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
2 * 130000, SDS-PAGE | Chlamydomonas reinhardtii |
235000 | - |
recombinant enzyme, native PAGE | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + oxidized ferredoxin 1 | Chlamydomonas reinhardtii | - |
acetyl-CoA + CO2 + reduced ferredoxin 1 + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 1 | Chlamydomonas reinhardtii CC124 | - |
acetyl-CoA + CO2 + reduced ferredoxin 1 + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 2 | Chlamydomonas reinhardtii | - |
acetyl-CoA + CO2 + reduced ferredoxin 2 + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 2 | Chlamydomonas reinhardtii CC124 | - |
acetyl-CoA + CO2 + reduced ferredoxin 2 + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Chlamydomonas reinhardtii CC124 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
when the recombinant enzyme (in 50 mM Tricine, pH 8.0) is exposed to air for 1 h on ice, a drastic loss of activity is observed | Chlamydomonas reinhardtii |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
using cytochrome c as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
2.3 | - |
using FMN as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
3.7 | - |
using methyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
4.5 | - |
using spinach ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
4.7 | - |
using methylene blue as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
6.3 | - |
using Chlamydomonas reinhardtii ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
12 | - |
using benzyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | neither NAD+ nor NADP+ can be reduced by the enzyme | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | neither NAD+ nor NADP+ can be reduced by the enzyme | Chlamydomonas reinhardtii CC124 | ? | - |
? | |
pyruvate + CoA + FMN | 5-6times lower activity with FMN as compared to benzyl viologen | Chlamydomonas reinhardtii | acetyl-CoA + CO2 + FMNH + H+ | - |
? | |
pyruvate + CoA + FMN | 5-6times lower activity with FMN as compared to benzyl viologen | Chlamydomonas reinhardtii CC124 | acetyl-CoA + CO2 + FMNH + H+ | - |
? | |
pyruvate + CoA + oxidized benzyl viologen | best activity with benzyl viologen | Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced benzyl viologen + H+ | - |
? | |
pyruvate + CoA + oxidized benzyl viologen | best activity with benzyl viologen | Chlamydomonas reinhardtii CC124 | acetyl-CoA + CO2 + reduced benzyl viologen + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 1 | - |
Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced ferredoxin 1 + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 1 | - |
Chlamydomonas reinhardtii CC124 | acetyl-CoA + CO2 + reduced ferredoxin 1 + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 2 | - |
Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced ferredoxin 2 + H+ | - |
? | |
pyruvate + CoA + oxidized ferredoxin 2 | - |
Chlamydomonas reinhardtii CC124 | acetyl-CoA + CO2 + reduced ferredoxin 2 + H+ | - |
? | |
pyruvate + CoA + oxidized methyl viologen | 3-4times lower activity with methyl viologen as compared to benzyl viologen | Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced methyl viologen + H+ | - |
? | |
pyruvate + CoA + oxidized methylene blue | 2.5-3times lower activity with methylene blue as compared to benzyl viologen | Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced methylene blue + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 130000, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
PFOR | - |
Chlamydomonas reinhardtii |
PFR | - |
Chlamydomonas reinhardtii |
pyruvate:ferredoxin oxidoreductase | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
- |
Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | recombinant enzyme, in 25 mM potassium phosphate | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | essential cofactor, upon addition of Mg2+, an ion that stabilizes thiamine diphosphate, the enzymatic activity almost doubles | Chlamydomonas reinhardtii |