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Literature summary for 1.2.7.1 extracted from

  • van Lis, R.; Baffert, C.; Coute, Y.; Nitschke, W.; Atteia, A.
    Chlamydomonas reinhardtii chloroplasts contain a homodimeric pyruvate:ferredoxin oxidoreductase that functions with FDX1 (2013), Plant Physiol., 161, 57-71.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information dithiothreitol has no influence on the activity Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
NaCl the addition of 0.3 M NaCl causes a significant inhibition (up to 80%) of the activity Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
oxidized ferredoxin 2 in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
0.0046
-
oxidized ferredoxin 1 in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
0.0052
-
CoA in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
0.16
-
pyruvate in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Chlamydomonas reinhardtii 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ upon addition of Mg2+, an ion that stabilizes thiamine diphosphate, the enzymatic activity almost doubles Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
2 * 130000, SDS-PAGE Chlamydomonas reinhardtii
235000
-
recombinant enzyme, native PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA + oxidized ferredoxin 1 Chlamydomonas reinhardtii
-
acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
?
pyruvate + CoA + oxidized ferredoxin 1 Chlamydomonas reinhardtii CC124
-
acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
?
pyruvate + CoA + oxidized ferredoxin 2 Chlamydomonas reinhardtii
-
acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
?
pyruvate + CoA + oxidized ferredoxin 2 Chlamydomonas reinhardtii CC124
-
acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii CC124
-
-
-

Oxidation Stability

Oxidation Stability Organism
when the recombinant enzyme (in 50 mM Tricine, pH 8.0) is exposed to air for 1 h on ice, a drastic loss of activity is observed Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
using cytochrome c as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
2.3
-
using FMN as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
3.7
-
using methyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
4.5
-
using spinach ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
4.7
-
using methylene blue as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
6.3
-
using Chlamydomonas reinhardtii ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
12
-
using benzyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information neither NAD+ nor NADP+ can be reduced by the enzyme Chlamydomonas reinhardtii ?
-
?
additional information neither NAD+ nor NADP+ can be reduced by the enzyme Chlamydomonas reinhardtii CC124 ?
-
?
pyruvate + CoA + FMN 5-6times lower activity with FMN as compared to benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + FMNH + H+
-
?
pyruvate + CoA + FMN 5-6times lower activity with FMN as compared to benzyl viologen Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + FMNH + H+
-
?
pyruvate + CoA + oxidized benzyl viologen best activity with benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced benzyl viologen + H+
-
?
pyruvate + CoA + oxidized benzyl viologen best activity with benzyl viologen Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced benzyl viologen + H+
-
?
pyruvate + CoA + oxidized ferredoxin 1
-
Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
?
pyruvate + CoA + oxidized ferredoxin 1
-
Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
?
pyruvate + CoA + oxidized ferredoxin 2
-
Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
?
pyruvate + CoA + oxidized ferredoxin 2
-
Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
?
pyruvate + CoA + oxidized methyl viologen 3-4times lower activity with methyl viologen as compared to benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced methyl viologen + H+
-
?
pyruvate + CoA + oxidized methylene blue 2.5-3times lower activity with methylene blue as compared to benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced methylene blue + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 130000, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
PFOR
-
Chlamydomonas reinhardtii
PFR
-
Chlamydomonas reinhardtii
pyruvate:ferredoxin oxidoreductase
-
Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
44
-
-
Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 recombinant enzyme, in 25 mM potassium phosphate Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate essential cofactor, upon addition of Mg2+, an ion that stabilizes thiamine diphosphate, the enzymatic activity almost doubles Chlamydomonas reinhardtii