Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and BL21(DE3)pLysS cells | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46270 | - |
unlipoylated enzyme, mass spectrometric analysis | Thermoplasma acidophilum |
46460 | - |
lipoylated enzyme, mass spectrometric analysis | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Bind affinity chromatography and Q-Sepharose Hi-Trap column chromatography | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Thermoplasma acidophilum | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 | - |
? | |
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Thermoplasma acidophilum | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2 | - |
? | |
4-methyl-2-oxopentanoate + NAD+ + CoA | - |
Thermoplasma acidophilum | 3-methylbutanoyl-CoA + CO2 + NADH | - |
? | |
pyruvate + NAD+ + CoA | - |
Thermoplasma acidophilum | acetyl-CoA + CO2 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoyl acyl-transferase | E2 component of the branched-chain 2-oxoacid dehydrogenase complex | Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoplasma acidophilum |