Any feedback?
Literature summary for 1.2.4.4 extracted from
- Purification, resolution, and reconstitution of branched-chain 2-keto acid dehydrogenase complex from bovine kidney (1988), Methods Enzymol., 166, 303-308.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE | Bos taurus |
| 46000 | - |
alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bos taurus | the activity of the multienzyme complex is regulated by reversible phosphorylation of the alpha-subunit of the E1 component, EC 1.2.4.4. | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, after concentration and dialysis into 50 mM Tris-HCl buffer, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM benzamidine, 1 mM PMSF, 1 mM DTT, pH 7.3, multienzyme complex is stable for at least 2 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the activity of the multienzyme complex is regulated by reversible phosphorylation of the alpha-subunit of the E1 component, EC 1.2.4.4. | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Bos taurus | |
| NAD+ | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
