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Literature summary for 1.2.4.1 extracted from

  • Kohda, Y.; Umeki, M.; Kono, T.; Terasaki, F.; Matsumura, H.; Tanaka, T.
    Thiamine ameliorates diabetes-induced inhibition of pyruvate dehydrogenase (PDH) in rat heart mitochondria: investigating the discrepancy between PDH activity and PDH E1alpha phosphorylation in cardiac fibroblasts exposed to high glucose (2010), J. Pharmacol. Sci., 113, 343-352.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-glucose treatment of cardiac fibroblasts with 35 mM D-glucose for 72 h reduces the PDH activity remarkably. 0.2 mM thiamine dramatically recovers the high glucose-induced PDH inhibition Rattus norvegicus
additional information there is no significant change in the PDH activity in the presence of D-mannitol (35 mM) Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the O-glycosylation of PDH E1alpha is involved in the regulation of the PDH activity Rattus norvegicus
phosphoprotein phosphorylation of the PDH E1alpha subunit by PDH kinase contributes to the suppression of PDH activity. Streptozotocin treatment causes a significant increase in the level of the phosphorylated PDH E1alpha subunit in the diabetic rat hearts Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
cardiac fibroblast
-
Rattus norvegicus
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heart
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
Rattus norvegicus acetyl-CoA + CO2 + NADH
-
?

Synonyms

Synonyms Comment Organism
PDH
-
Rattus norvegicus
PDH E1alpha subunit Rattus norvegicus
pyruvate dehydrogenase complex
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
thiamine diphosphate
-
Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus the activity of pyruvate dehydrogenase is reduced in diabetic rats. Thiamine ameliorates diabetes-induced PDH inhibition by suppressing the increased expression of the O-glycosylated protein down
Rattus norvegicus high glucose loads do not alter the level of PDH mRNA expressions in cardiac fibroblasts additional information
Rattus norvegicus the O-glycosylated PDH protein is markedly increased in cardiac fibroblasts exposed to high glucose up