Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | treatment of cardiac fibroblasts with 35 mM D-glucose for 72 h reduces the PDH activity remarkably. 0.2 mM thiamine dramatically recovers the high glucose-induced PDH inhibition | Rattus norvegicus | |
additional information | there is no significant change in the PDH activity in the presence of D-mannitol (35 mM) | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the O-glycosylation of PDH E1alpha is involved in the regulation of the PDH activity | Rattus norvegicus |
phosphoprotein | phosphorylation of the PDH E1alpha subunit by PDH kinase contributes to the suppression of PDH activity. Streptozotocin treatment causes a significant increase in the level of the phosphorylated PDH E1alpha subunit in the diabetic rat hearts | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac fibroblast | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + NAD+ | - |
Rattus norvegicus | acetyl-CoA + CO2 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Rattus norvegicus |
PDH E1alpha | subunit | Rattus norvegicus |
pyruvate dehydrogenase complex | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
thiamine diphosphate | - |
Rattus norvegicus |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | the activity of pyruvate dehydrogenase is reduced in diabetic rats. Thiamine ameliorates diabetes-induced PDH inhibition by suppressing the increased expression of the O-glycosylated protein | down |
Rattus norvegicus | high glucose loads do not alter the level of PDH mRNA expressions in cardiac fibroblasts | additional information |
Rattus norvegicus | the O-glycosylated PDH protein is markedly increased in cardiac fibroblasts exposed to high glucose | up |