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Literature summary for 1.2.4.1 extracted from
- Amino-terminal residues 1-45 of the Escherichia coli pyruvate dehydrogenase complex E1 subunit interact with the E2 subunit and are required for activity of the complex but not for reductive acetylation of the E2 subunit (2004), Biochemistry, 43, 14037-14046.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D15A | site-directed mutagenesis, 0.19% remaining activity with NAD+ and 16% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
| D7A | site-directed mutagenesis, 0.14% remaining activity with NAD+ and 63% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
| D9A | site-directed mutagenesis, inactive with NAD+, 80% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
| E12D | site-directed mutagenesis, 2.3% remaining activity with NAD+ and 90% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
| E12Q | site-directed mutagenesis, 3.3% remaining activity with NAD+ and 59% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
| I11A | site-directed mutagenesis, 94% remaining activity with NAD+ and E1 activity with 2,6-dichlorophenolindophenol as electron acceptor is like the wild-type E1 activity | Escherichia coli |
| additional information | construction of several N-terminal E1 deletion mutants by tryptic or chymotryptic digest all showing reduced enzyme activity | Escherichia coli |
| P10A | site-directed mutagenesis, unaltered activity with NAD+ and 50% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
| R14A | site-directed mutagenesis, 0.25% remaining activity with NAD+ as electron acceptor, E1 activity with 2,6-dichlorophenolindophenol is like the wild-type E1 activity | Escherichia coli |
| T13A | site-directed mutagenesis, 13% remaining activity with NAD+ and 26% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99474 | - |
x * 99474, E1, laser light scattering | Escherichia coli |
| 169000 | - |
whole pyruvate dehydrogenase enzyme complex, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | overall reaction of the multienzyme complex, overview | ? | - |
? | |
| pyruvate + CoA + NAD+ | Escherichia coli | - |
acetyl-CoA + CO2 + NADH | - |
? | |
| pyruvate + [dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine | Escherichia coli | - |
[dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.385 | - |
purified recombinant wild-type E1, with 2,6-dichlorophenolindophenol as electron acceptor | Escherichia coli |
| 28.35 | - |
purified recombinant wild-type E1, with NAD+ as electron acceptor | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | overall reaction of the multienzyme complex, overview | Escherichia coli | ? | - |
? | |
| additional information | N-terminal residues 1-45 of the pyruvate dehydrogenase complex E1 subunit interact with the E2 subunit and are required for activity of the complex but not for reductive acetylation of the E2 subunit | Escherichia coli | ? | - |
? | |
| pyruvate + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + CO2 + NADH | - |
? | |
| pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol | artificial electron acceptor | Escherichia coli | acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| pyruvate + [dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine | - |
Escherichia coli | [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 99474, E1, laser light scattering | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | E1 is a component of the pyruvate dehydrogenase multienzyme complex PDHc | Escherichia coli |
| PDHc-E1 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| thiamine diphosphate | - |
Escherichia coli | |
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