Protein Variants | Comment | Organism |
---|---|---|
D15A | site-directed mutagenesis, 0.19% remaining activity with NAD+ and 16% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
D7A | site-directed mutagenesis, 0.14% remaining activity with NAD+ and 63% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
D9A | site-directed mutagenesis, inactive with NAD+, 80% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
E12D | site-directed mutagenesis, 2.3% remaining activity with NAD+ and 90% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
E12Q | site-directed mutagenesis, 3.3% remaining activity with NAD+ and 59% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
I11A | site-directed mutagenesis, 94% remaining activity with NAD+ and E1 activity with 2,6-dichlorophenolindophenol as electron acceptor is like the wild-type E1 activity | Escherichia coli |
additional information | construction of several N-terminal E1 deletion mutants by tryptic or chymotryptic digest all showing reduced enzyme activity | Escherichia coli |
P10A | site-directed mutagenesis, unaltered activity with NAD+ and 50% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
R14A | site-directed mutagenesis, 0.25% remaining activity with NAD+ as electron acceptor, E1 activity with 2,6-dichlorophenolindophenol is like the wild-type E1 activity | Escherichia coli |
T13A | site-directed mutagenesis, 13% remaining activity with NAD+ and 26% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99474 | - |
x * 99474, E1, laser light scattering | Escherichia coli |
169000 | - |
whole pyruvate dehydrogenase enzyme complex, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | overall reaction of the multienzyme complex, overview | ? | - |
? | |
pyruvate + CoA + NAD+ | Escherichia coli | - |
acetyl-CoA + CO2 + NADH | - |
? | |
pyruvate + [dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine | Escherichia coli | - |
[dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.385 | - |
purified recombinant wild-type E1, with 2,6-dichlorophenolindophenol as electron acceptor | Escherichia coli |
28.35 | - |
purified recombinant wild-type E1, with NAD+ as electron acceptor | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | overall reaction of the multienzyme complex, overview | Escherichia coli | ? | - |
? | |
additional information | N-terminal residues 1-45 of the pyruvate dehydrogenase complex E1 subunit interact with the E2 subunit and are required for activity of the complex but not for reductive acetylation of the E2 subunit | Escherichia coli | ? | - |
? | |
pyruvate + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + CO2 + NADH | - |
? | |
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol | artificial electron acceptor | Escherichia coli | acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
pyruvate + [dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine | - |
Escherichia coli | [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 99474, E1, laser light scattering | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | E1 is a component of the pyruvate dehydrogenase multienzyme complex PDHc | Escherichia coli |
PDHc-E1 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
thiamine diphosphate | - |
Escherichia coli |