Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.3.7 extracted from

  • Zarepour, M.; Simon, K.; Wilch, M.; Nielaender, U.; Koshiba, T.; Seo, M.; Lindel, T.; Bittner, F.
    Identification of superoxide production by Arabidopsis thaliana aldehyde oxidases AAO1 and AAO3 (2012), Plant Mol. Biol., 80, 659-671.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
diphenylene iodonium strong inhibitor Arabidopsis thaliana
KCN complete inhibition at 20 mM after 1 h incubation at room temperature Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
indole-3-acetaldehyde + O2 Arabidopsis thaliana highly efficient conversion indole-3-acetate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q7G193
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and Source 15Q colum chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.093
-
using heptaldehyde as substrate, pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.11
-
using benzaldehyde as substrate, pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.515
-
using abscisic aldehyde as substrate, pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.558
-
using indole-3-carbaldehyde as substrate, pH 7.5, temperature not specified in the publication Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
abscisic aldehyde + oxidized 2,6-dichloroindophenol + H2O
-
Arabidopsis thaliana abscisic acid + reduced 2,6-dichloroindophenol
-
?
benzaldehyde + oxidized 2,6-dichloroindophenol + H2O weak substrate Arabidopsis thaliana benzoic acid + reduced 2,6-dichloroindophenol
-
?
heptaldehyde + oxidized 2,6-dichloroindophenol + H2O weak substrate Arabidopsis thaliana heptanoic acid + reduced 2,6-dichloroindophenol
-
?
indole-3-acetaldehyde + O2 highly efficient conversion Arabidopsis thaliana indole-3-acetate + H2O2
-
?
indole-3-acetaldehyde + oxidized 2,6-dichloroindophenol + H2O efficient substrate Arabidopsis thaliana indole-3-acetate + reduced 2,6-dichloroindophenol
-
?
additional information in addition to the two-electron reduction of molecular oxygen, isoform AAO1 also catalyzes a one-electron transfer to molecular oxygen, leading to the formation of O2-. However, the production of O2 appears to be less efficient than the production of H2O2 Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AAO1
-
Arabidopsis thaliana
AOgamma
-
Arabidopsis thaliana