Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphenylene iodonium | strong inhibitor | Arabidopsis thaliana | |
KCN | complete inhibition at 20 mM after 1 h incubation at room temperature | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-acetaldehyde + O2 | Arabidopsis thaliana | highly efficient conversion | indole-3-acetate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q7G193 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, and Source 15Q colum chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.093 | - |
using heptaldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
0.11 | - |
using benzaldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
0.515 | - |
using abscisic aldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
0.558 | - |
using indole-3-carbaldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
abscisic aldehyde + oxidized 2,6-dichloroindophenol + H2O | - |
Arabidopsis thaliana | abscisic acid + reduced 2,6-dichloroindophenol | - |
? | |
benzaldehyde + oxidized 2,6-dichloroindophenol + H2O | weak substrate | Arabidopsis thaliana | benzoic acid + reduced 2,6-dichloroindophenol | - |
? | |
heptaldehyde + oxidized 2,6-dichloroindophenol + H2O | weak substrate | Arabidopsis thaliana | heptanoic acid + reduced 2,6-dichloroindophenol | - |
? | |
indole-3-acetaldehyde + O2 | highly efficient conversion | Arabidopsis thaliana | indole-3-acetate + H2O2 | - |
? | |
indole-3-acetaldehyde + oxidized 2,6-dichloroindophenol + H2O | efficient substrate | Arabidopsis thaliana | indole-3-acetate + reduced 2,6-dichloroindophenol | - |
? | |
additional information | in addition to the two-electron reduction of molecular oxygen, isoform AAO1 also catalyzes a one-electron transfer to molecular oxygen, leading to the formation of O2-. However, the production of O2 appears to be less efficient than the production of H2O2 | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AAO1 | - |
Arabidopsis thaliana |
AOgamma | - |
Arabidopsis thaliana |