Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Gelatoporia subvermispora |
General Stability | Organism |
---|---|
enzyme activity of the C isoform is lost in the presence of sodium dodecyl sulfate (SDS) | Gelatoporia subvermispora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Soluble and active protein obtained only when the enzyme is coexpressed with the chaperones DnaK and DnaJ (G isoform) and when a manganese salt is added to the growth medium (C and G isoform) | Gelatoporia subvermispora | |
SDS | enzyme activity of the C isoform is lost in the presence of sodium dodecyl sulfate | Gelatoporia subvermispora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49800 | - |
C isoform, calculated from protein sequence | Gelatoporia subvermispora |
56000 | - |
G isoform, SDS-PAGE | Gelatoporia subvermispora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | - |
ATCC 90466, oxalate oxidase allelic isoforms C and G | - |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography, pH precipitation, phosphocellulose chromatography, gel filtration | Gelatoporia subvermispora |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.2 | - |
C isoform | Gelatoporia subvermispora |
20 | - |
G isoform | Gelatoporia subvermispora |