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Literature summary for 1.2.3.4 extracted from

  • Satyapal; Pundir, C.S.
    Purification and properties of an oxalate oxidase from leaves of grain sorghum hybrid CSH-5 (1993), Biochim. Biophys. Acta, 1161, 1-5.
    View publication on PubMed
Search for more literature for 1.2.3.4

Activating Compound

Activating Compound Comment Organism Structure
Cu2+ Cu2+ + FMN 0.5 mM 280% activation Sorghum sp.
Cu2+ Cu2+ + diethyldithiocarbamate 0.5 mM 60% activation Sorghum sp.
Cu2+ Cu2+ + FAD 0.5 mM 250% activation Sorghum sp.
Cu2+ Cu2+ + L-cysteine 0.5 mM 46% activation Sorghum sp.
Cu2+ Cu2+ + 2-mercaptoethanol 0.5 mM 62% activation Sorghum sp.
Cu2+ Cu2+ + 8-hydroxyquinoline 0.5 mM 100% activation Sorghum sp.
CuSO4 0.5 mM concentration 200% activation Sorghum sp.
FAD 0.5 mM concentration 31% activation Sorghum sp.
FMN 0.5 mM concentration 57% activation Sorghum sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.5 mM concentration 40% inhibition Sorghum sp.
8-hydroxyquinoline 0.5 mM concentration 56% activity retained Sorghum sp.
alpha,alpha'-dipyridyl 0.5 mM concentration 26% inhibition Sorghum sp.
azide 0.5 mM concentration 57% inhibition Sorghum sp.
diethyldithiocarbamate 0.5 mM concentration 64% activity retained Sorghum sp.
Dithionite 0.5 mM concentration 42% inhibition Sorghum sp.
EDTA 0.5 mM concentration 64% activity retained Sorghum sp.
Iodide 0.5 mM concentration 14% inhibition Sorghum sp.
L-cysteine 0.5 mM concentration 60% inhibition Sorghum sp.
SrCl2 slight inhibition at 0.5 mM concentration Sorghum sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Sorghum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 alpha2, 2 * 62000, SDS-PAGE Sorghum sp.
120200
-
 gel filtration Sorghum sp.

Organism

Organism UniProt Comment Textmining
Sorghum sp.
-
-
-
Sorghum sp. CSH-5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sorghum sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Sorghum sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.39
-
 H2O2 production Sorghum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalic acid + O2 + 2 H+
-
 Sorghum sp. 2 CO2 + H2O2
-
 ir
oxalic acid + O2 + 2 H+
-
 Sorghum sp. CSH-5 2 CO2 + H2O2
-
 ir

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 62000, SDS-PAGE Sorghum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Sorghum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Sorghum sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 7 50% activity at pH 4.0 and 7.0 Sorghum sp.