Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose | physiological concentration, 20% activation free protein, 25% activation immobilized protein | Amaranthus spinosus | |
glycine | 1 mM concentration, 10% activation free protein, 18% activation immobilized protein | Amaranthus spinosus | |
hippuric acid | 1 mM concentration, 10% activation free protein, 8% activation immobilized protein | Amaranthus spinosus | |
serine | 1 mM concentration, 12% activation free protein, 10% activation immobilized protein | Amaranthus spinosus | |
Urea | physiological concentration, 8% activation free protein | Amaranthus spinosus | |
uric acid | 1 mM concentration, 12% activation free protein, 8% activation immobilized protein | Amaranthus spinosus |
Application | Comment | Organism |
---|---|---|
medicine | immobilized oxalate oxidase to determine oxalate concentration in urine for diagnosis of various forms of hyperoxaluria | Amaranthus spinosus |
General Stability | Organism |
---|---|
immobilized on alkylamine glass | Amaranthus spinosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | wild type and immobilized 88% and 81% inhibition respectively | Amaranthus spinosus | |
Dithionite | - |
Amaranthus spinosus | |
glutathione | slight inhibition, wild type and immobilized | Amaranthus spinosus | |
L-cysteine | wild type and immobilized slight inhibition | Amaranthus spinosus | |
NaCl | 10% inhibition at 1 mM concentration | Amaranthus spinosus | |
nitrate | mM concentration | Amaranthus spinosus | |
nitrite | 1 mM concentration 10% inhibition | Amaranthus spinosus | |
pyruvate | 1 mM concentration 15% inhibition of the free enzyme, not of the immobilized enzyme | Amaranthus spinosus | |
salicylate | - |
Amaranthus spinosus | |
ZnSO4 | 1 mM concentration, 10% inhibition of the free enzyme, not of the immobilized enzyme | Amaranthus spinosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
oxalate | - |
Amaranthus spinosus | |
0.39 | - |
oxalate | immobilized protein | Amaranthus spinosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Amaranthus spinosus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amaranthus spinosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Amaranthus spinosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Amaranthus spinosus | - |
Storage Stability | Organism |
---|---|
4°C, 3 months, distilled water, free protein, complete loss of activity, immobilized protein, 70% of activity retained | Amaranthus spinosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalic acid + O2 + 2 H+ | - |
Amaranthus spinosus | 2 CO2 + H2O2 | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
immobilized protein | Amaranthus spinosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
after 30 min wild type 60% activity, immobilized protein 27% activity | Amaranthus spinosus |