Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris X33 | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | sub mM concentration | Hordeum vulgare | |
Iodide | sub mM concentration | Hordeum vulgare | |
nitrite | sub mM concentration | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Hordeum vulgare | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.015 g per mol protein | Hordeum vulgare | |
Fe2+ | 0.015 g per mol protein | Hordeum vulgare | |
Mn2+ | 0.201 g per monomer, 6-coordination | Hordeum vulgare | |
Zn2+ | 0.36-0.62 g per mol protein | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
alpha6, 6 * 26000, SDS-PAGE | Hordeum vulgare |
140000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalic acid + O2 + 2 H+ | Hordeum vulgare | role in plant signaling and defense | 2 CO2 + H2O2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Oxidation Stability | Organism |
---|---|
by treatment with K3Fe(CN)6 decreases activity, desalting restores initial activity | Hordeum vulgare |
resistant against Na2IrCl6 | Hordeum vulgare |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3 kDa carbohydrate per 1 mol protein | Hordeum vulgare |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-180°C, several months, no activity loss | Hordeum vulgare |
8°C, several months, no activity loss, conversion in 4 months to 23 kDa protein, loss of carbohydrate | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalic acid + O2 + 2 H+ | - |
Hordeum vulgare | 2 CO2 + H2O2 | - |
ir | |
oxalic acid + O2 + 2 H+ | role in plant signaling and defense | Hordeum vulgare | 2 CO2 + H2O2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha6, 6 * 26000, SDS-PAGE | Hordeum vulgare |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermally extremely stable | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Hordeum vulgare |