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Literature summary for 1.2.3.4 extracted from

  • Whittaker, M.M.; Whittaker, J.W.
    Characterization of recombinant barley oxalate oxidase expressed by Pichia pastoris (2002), J. Biol. Inorg. Chem., 7, 136-145.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X33 Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
fluoride sub mM concentration Hordeum vulgare
Iodide sub mM concentration Hordeum vulgare
nitrite sub mM concentration Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Hordeum vulgare 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 0.015 g per mol protein Hordeum vulgare
Fe2+ 0.015 g per mol protein Hordeum vulgare
Mn2+ 0.201 g per monomer, 6-coordination Hordeum vulgare
Zn2+ 0.36-0.62 g per mol protein Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
alpha6, 6 * 26000, SDS-PAGE Hordeum vulgare
140000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalic acid + O2 + 2 H+ Hordeum vulgare role in plant signaling and defense 2 CO2 + H2O2
-
ir

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Oxidation Stability

Oxidation Stability Organism
by treatment with K3Fe(CN)6 decreases activity, desalting restores initial activity Hordeum vulgare
resistant against Na2IrCl6 Hordeum vulgare

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3 kDa carbohydrate per 1 mol protein Hordeum vulgare

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare

Storage Stability

Storage Stability Organism
-180°C, several months, no activity loss Hordeum vulgare
8°C, several months, no activity loss, conversion in 4 months to 23 kDa protein, loss of carbohydrate Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalic acid + O2 + 2 H+
-
Hordeum vulgare 2 CO2 + H2O2
-
ir
oxalic acid + O2 + 2 H+ role in plant signaling and defense Hordeum vulgare 2 CO2 + H2O2
-
ir

Subunits

Subunits Comment Organism
hexamer alpha6, 6 * 26000, SDS-PAGE Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermally extremely stable Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Hordeum vulgare