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Literature summary for 1.2.3.1 extracted from

  • Choughule, K.V.; Joswig-Jones, C.A.; Jones, J.P.
    Interspecies differences in the metabolism of methotrexate: An insight into the active site differences between human and rabbit aldehyde oxidase (2015), Biochem. Pharmacol., 96, 288-295.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.3.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
-
 Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
A1023Y significant decrease in activity on methotrexate Homo sapiens
I1085A slightly higher activity on methotrexate compared to the wild type Homo sapiens
I1085A/V1016R significant decrease in activity compared to the wild type and I1085A mutant Homo sapiens
I11085A/A1023Y catalytic turnover of methotrexate is similar to the I1085A single mutant Homo sapiens
additional information substitution of nucleotides of human AOX1 with relevant ones of rabbit AOX1 Homo sapiens
V1016R significant decrease in activity on methotrexate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 methotrexate pH 7.5, 37°C Oryctolagus cuniculus
2.792
-
 methotrexate pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q06278
-
-
Oryctolagus cuniculus O54754
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methotrexate + H2O + O2
-
 Homo sapiens 7-hydroxymethotrexate + H2O2
-
 ?
methotrexate + H2O + O2
-
 Oryctolagus cuniculus 7-hydroxymethotrexate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
AOX1
-
 Homo sapiens
AOX1
-
 Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 methotrexate pH 7.5, 37°C Homo sapiens
84
-
 methotrexate pH 7.5, 37°C Oryctolagus cuniculus

General Information

General Information Comment Organism
physiological function the amount of AOX1 in human liver is similar to rabbit liver, while the metabolism of methotrexate in rabbit liver cytosol is several orders of magnitude higher than any of the other species tested Homo sapiens
physiological function the amount of AOX1 in human liver is similar to rabbit liver, while the metabolism of methotrexate in rabbit liver cytosol is several orders of magnitude higher than any of the other species tested Oryctolagus cuniculus