Literature summary for 1.2.3.1 extracted from

Mahro, M.; Coelho, C.; Trincao, J.; Rodrigues, D.; Terao, M.; Garattini, E.; Saggu, M.; Lendzian, F.; Hildebrandt, P.; Romao, M.J.; Leimkuehler, S.
Characterization and crystallization of mouse aldehyde oxidase 3: from mouse liver to Escherichia coli heterologous protein expression (2011), Drug Metab. Dispos., 39, 1939-1945.

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA of mAOX3 cloned from mouse CD1 liver into pMMA1 plasmid and expression as an N-terminal fusion protein with a His6 tag, coexpression with mMCSF, heterologous expression in Escherichia coli	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vast majority of the crystallization trials performed using the recombinant protein, usable data set of crystals of native mAOX3 with a resolution of 2.9 A	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.013	-	benzaldehyde	apparent Km-value of native mAOX3, pH 7.4, 25°C	Mus musculus
0.02	-	benzaldehyde	apparent Km-value of recombinant mAOX3, pH 7.4, 25°C	Mus musculus
0.026	-	Butanal	apparent Km-value of recombinant mAOX3, pH 7.4, 25°C	Mus musculus
0.029	-	Butanal	apparent Km-value of native mAOX3, pH 7.4, 25°C	Mus musculus
0.097	-	2-hydroxypyrimidine	apparent Km-value of recombinant mAOX3, pH 7.4, 25°C	Mus musculus
0.173	-	2-hydroxypyrimidine	apparent Km-value of native mAOX3, pH 7.4, 25°C	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	SDS-PAGE, native and recombinant protein, degradation products of 130000, 80000, 70000, and 55000 Da in case of purified recombinant mAOX3	Mus musculus
300000	-	gel filtration, native and recombinant mAOX3	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mAOX3 purified using sequential Ni-NTA chromatography and size exclusion chromatography, a chemical sulfuration step performed to further increase the activity of the enzyme 1.4fold and after coexpression with mMCSF and chemical sulfuration, 30% of recombinant mAOX3 exists in the catalytically active form, native mAOX3 purified by ammonium sulfate precipitation with 50% saturation, benzamidine Sepharose chromatography and a linear NaCl gradient on a 5/5 FPLC Mono Q column	Mus musculus

Purification (Comment)

Organism

recombinant mAOX3 purified using sequential Ni-NTA chromatography and size exclusion chromatography, a chemical sulfuration step performed to further increase the activity of the enzyme 1.4fold and after coexpression with mMCSF and chemical sulfuration, 30% of recombinant mAOX3 exists in the catalytically active form, native mAOX3 purified by ammonium sulfate precipitation with 50% saturation, benzamidine Sepharose chromatography and a linear NaCl gradient on a 5/5 FPLC Mono Q column

Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2-hydroxypyrimidine + H2O + ferricyanide + O2	-	Mus musculus	?	-	?
benzaldehyde + 2 ferricyanide + H2O	-	Mus musculus	benzoate + 2 ferrocyanide + 2 H+	-	?
butanal + 2 ferricyanide + H2O	-	Mus musculus	butanoate + 2 ferrocyanide + 2 H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 150000, gel filtration	Mus musculus

Synonyms

Synonyms	Comment	Organism
mAOX3	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.733	-	benzaldehyde	apparent Kcat-value of recombinant mAOX3, pH 7.4, 25°C	Mus musculus
2.167	-	benzaldehyde	apparent Kcat-value of native mAOX3, pH 7.4, 25°C	Mus musculus
2.333	-	Butanal	apparent Kcat-value of recombinant mAOX3, pH 7.4, 25°C	Mus musculus
6.4	-	Butanal	apparent Kcat-value of native mAOX3, pH 7.4, 25°C	Mus musculus
6.88	-	2-hydroxypyrimidine	apparent Kcat-value of recombinant mAOX3, pH 7.4, 25°C	Mus musculus
21.32	-	2-hydroxypyrimidine	apparent Kcat-value of native mAOX3, pH 7.4, 25°C	Mus musculus

Cofactor

Cofactor	Comment	Organism
FAD	-	Mus musculus
molybdenum cofactor	i.e. MoCo	Mus musculus
[2Fe-2S]-center	two distinct ones	Mus musculus

General Information

General Information	Comment	Organism
physiological function	AOX3 is an enzyme of well known importance in drug metabolism and therefore of increasing importance in recent drug design programs	Mus musculus