Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.3.1 extracted from

  • Uchida, H.; Kondo, D.; Yamashita, A.; Nagaosa, Y.; Sakurai, T.; Fujii, Y.; Fujishiro, K.; Aisaka, K.; Uwajima, T.
    Purification and characterization of an aldehyde oxidase from Pseudomonas sp. KY 4690 (2003), FEMS Microbiol. Lett., 229, 31-36.
    View publication on PubMed
Search for more literature for 1.2.3.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 n-Heptylaldehyde 37°C, pH 7.0 Pseudomonas sp.
0.18
-
 n-Valeraldehyde 37°C, pH 7.0 Pseudomonas sp.
0.29
-
 Glutaraldehyde 37°C, pH 7.0 Pseudomonas sp.
0.31
-
 DL-glyceraldehyde 37°C, pH 7.0 Pseudomonas sp.
0.52
-
 benzaldehyde 37°C, pH 7.0 Pseudomonas sp.
1.3
-
 acetaldehyde 37°C, pH 7.0 Pseudomonas sp.
1.5
-
 Vanillin 37°C, pH 7.0 Pseudomonas sp.
8.5
-
 formaldehyde 37°C, pH 7.0 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron aldehyde oxidase probably contains 2 mol of [2Fe-2S] clusters per mol of enzyme Pseudomonas sp.
Molybdenum aldehyde oxidase probably contains 1 mol of molybdenum-molybdpterin-cytosine dinucleotide per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 1 * 88000 + 1 * 39000 + 1 * 18000, SDS-PAGE Pseudomonas sp.
39000
-
 1 * 88000 + 1 * 39000 + 1 * 18000, SDS-PAGE Pseudomonas sp.
88000
-
 1 * 88000 + 1 * 39000 + 1 * 18000, SDS-PAGE Pseudomonas sp.
132000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 KY 4690
-
Pseudomonas sp. KY 4690
-
 KY 4690
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment, Butyl-toyopearl, Resource Q Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + O2 + H2O
-
 Pseudomonas sp. acetate + H2O2
-
 ?
acetaldehyde + O2 + H2O
-
 Pseudomonas sp. KY 469 acetate + H2O2
-
 ?
acetaldehyde + O2 + H2O
-
 Pseudomonas sp. KY 4690 acetate + H2O2
-
 ?
benzaldehyde + O2 + H2O
-
 Pseudomonas sp. benzoate + H2O2
-
 ?
benzaldehyde + O2 + H2O
-
 Pseudomonas sp. KY 469 benzoate + H2O2
-
 ?
benzaldehyde + O2 + H2O
-
 Pseudomonas sp. KY 4690 benzoate + H2O2
-
 ?
DL-glyceraldehyde + O2 + H2O
-
 Pseudomonas sp. glycerate + H2O2
-
 ?
DL-glyceraldehyde + O2 + H2O
-
 Pseudomonas sp. KY 469 glycerate + H2O2
-
 ?
DL-glyceraldehyde + O2 + H2O
-
 Pseudomonas sp. KY 4690 glycerate + H2O2
-
 ?
formaldehyde + O2 + H2O
-
 Pseudomonas sp. formate + H2O2
-
 ?
formaldehyde + O2 + H2O
-
 Pseudomonas sp. KY 469 formate + H2O2
-
 ?
formaldehyde + O2 + H2O
-
 Pseudomonas sp. KY 4690 formate + H2O2
-
 ?
glutaraldehyde + O2 + H2O
-
 Pseudomonas sp. glutarate + H2O2
-
 ?
n-heptylaldehyde + O2 + H2O
-
 Pseudomonas sp. heptanoate + H2O2
-
 ?
n-valeraldehyde + O2 + H2O
-
 Pseudomonas sp. valerate + H2O2
-
 ?
vanillin + O2 + H2O
-
 Pseudomonas sp. vanillic acid + H2O2
-
 ?
vanillin + O2 + H2O
-
 Pseudomonas sp. KY 469 vanillic acid + H2O2
-
 ?
vanillin + O2 + H2O
-
 Pseudomonas sp. KY 4690 vanillic acid + H2O2
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 88000 + 1 * 39000 + 1 * 18000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 no loss of activity after 30 min at pH 7.0 Pseudomonas sp.
80
-
 5% loss of activity after 30 min at pH 7.0 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 no loss of activity after 30 min at 70°C Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD aldehyde oxidase probably contains 1 mol of FAD per mol of enzyme Pseudomonas sp.