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Literature summary for 1.2.3.1 extracted from

  • Duarte, R.O.; Archer, M.; Dias, J.M.; Bursakov, S.; Huber, R.; Moura, I.; Romao, M.J.; Moura, J.J.G.
    Biochemical/spectroscopic characterization and preliminary x-ray analysis of a new aldehyde oxidoreductase isolated from Desulfovibrio desulfuricans ATCC 27774 (2000), Biochem. Biophys. Res. Commun., 268, 745-749.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
2.8 A resolution, 98% complete Desulfovibrio desulfuricans

Inhibitors

Inhibitors Comment Organism Structure
arsenite 65% inhibition at 20 mM Desulfovibrio desulfuricans
Isopropanol moderate inhibitor Desulfovibrio desulfuricans
nitrate moderate inhibitor Desulfovibrio desulfuricans
phosphate moderate inhibitor Desulfovibrio desulfuricans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
propionaldehyde
-
Desulfovibrio desulfuricans
0.105
-
butanaldehyde
-
Desulfovibrio desulfuricans
0.151
-
benzaldehyde
-
Desulfovibrio desulfuricans
0.25
-
acetaldehyde
-
Desulfovibrio desulfuricans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe/S center type I and Fe/S center type II Desulfovibrio desulfuricans
Mo5+
-
Desulfovibrio desulfuricans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
alpha2, 2 * 100000, SDS-PAGE Desulfovibrio desulfuricans
200000
-
gel filtration Desulfovibrio desulfuricans

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
ATCC 27774
-

Purification (Commentary)

Purification (Comment) Organism
-
Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
2,6-dichlorophenol-indophenol under aerobic conditions as electron acceptor Desulfovibrio desulfuricans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + O2 2,6-dichlorophenol-indophenol as electron acceptor Desulfovibrio desulfuricans acetic acid + H2O2
-
?
benzaldehyde + H2O + O2 2,6-dichlorophenol-indophenol also as electron acceptor Desulfovibrio desulfuricans benzoic acid + H2O2
-
?
butanal + H2O + O2 2,6-dichlorophenol-indophenol as electron acceptor Desulfovibrio desulfuricans butyric acid + H2O2
-
?
propionaldehyde + H2O + O2 2,6-dichlorophenol-indophenol as electron acceptor Desulfovibrio desulfuricans propionic acid + H2O2
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 100000, SDS-PAGE Desulfovibrio desulfuricans

Synonyms

Synonyms Comment Organism
AOR
-
Desulfovibrio desulfuricans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Desulfovibrio desulfuricans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.9 9.5
-
Desulfovibrio desulfuricans

Cofactor

Cofactor Comment Organism Structure
molybdopterin cytosine dinucleotide
-
Desulfovibrio desulfuricans