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Literature summary for 1.2.2.B1 extracted from

  • Zahn, J.A.; Bergmann, D.J.; Boyd, J.M.; Kunz, R.C.; DiSpirito, A.A.
    Membrane-associated quinoprotein formaldehyde dehydrogenase from Methylococcus capsulatus bath (2001), J. Bacteriol., 183, 6832-6840.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.2.B1

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ partial inhibition Methylococcus capsulatus
Fe2+ partial inhibition Methylococcus capsulatus
additional information the enzyme is insensitive to EDTA, N-ethylmaleimide, cyanide and azide Methylococcus capsulatus
phenylhydrazine
-
 Methylococcus capsulatus
Zn2+ partial inhibition Methylococcus capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.143
-
 formaldehyde 20°C, pH 7.4 Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Methylococcus capsulatus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49500
-
 4 * 49500, SDS-PAGE Methylococcus capsulatus
210000
-
 gel filtration Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + ferricytochrome b559/569 complex Methylococcus capsulatus the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium formate + ferrocytochrome b559/569 complex
-
 ?
formaldehyde + ferricytochrome b559/569 complex Methylococcus capsulatus Bath the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium formate + ferrocytochrome b559/569 complex
-
 ?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus Bath
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylococcus capsulatus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:high copper medium-grown cell
-
 Methylococcus capsulatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
146
-
-
 Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + dichloroindophenol formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol Methylococcus capsulatus formate + reduced dichloroindophenol
-
 ?
formaldehyde + dichloroindophenol formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol Methylococcus capsulatus Bath formate + reduced dichloroindophenol
-
 ?
formaldehyde + ferricytochrome b559/569 + H2O
-
 Methylococcus capsulatus formate + ferrocytochrome b559/569 + 2 H+
-
 r
formaldehyde + ferricytochrome b559/569 + H2O
-
 Methylococcus capsulatus Bath formate + ferrocytochrome b559/569 + 2 H+
-
 r
formaldehyde + ferricytochrome b559/569 complex
-
 Methylococcus capsulatus formate + ferrocytochrome b559/569 complex
-
 ?
formaldehyde + ferricytochrome b559/569 complex the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium Methylococcus capsulatus formate + ferrocytochrome b559/569 complex
-
 ?
formaldehyde + ferricytochrome b559/569 complex
-
 Methylococcus capsulatus Bath formate + ferrocytochrome b559/569 complex
-
 ?
formaldehyde + ferricytochrome b559/569 complex the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium Methylococcus capsulatus Bath formate + ferrocytochrome b559/569 complex
-
 ?
additional information no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde Methylococcus capsulatus ?
-
 ?
additional information no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde Methylococcus capsulatus Bath ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 49500, SDS-PAGE Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
DL-FalDH
-
 Methylococcus capsulatus
formaldehyde-cytochrome b559/569 oxidoreductase
-
 Methylococcus capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
266
-
 ferricytochrome b559/569 20°C, pH 7.4 Methylococcus capsulatus
290
-
 dichloroindophenol 20°C, pH 7.4 Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone pyrroloquinoline quinone-to-subunit stochiometry of approximately 1:1, 3.6 mol of pyrroloquinoline quinone per mol of enzyme Methylococcus capsulatus