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Literature summary for 1.2.1.97 extracted from

  • Felux A.-K, F.A.; Spiteller D, S.D.; Klebensberger J, K.J.; Schleheck D, S.D.
    Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1 (2015), Proc. Natl. Acad. Sci. USA, 112, E4298-E4305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.97

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 (2S)-3-sulfolactaldehyde with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
0.14
-
 (2S)-3-sulfolactaldehyde with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-3-sulfolactaldehyde + NAD+ + H2O Pseudomonas putida
-
 (2S)-3-sulfolactate + NADH + H+
-
 ?
(2S)-3-sulfolactaldehyde + NAD+ + H2O Pseudomonas putida SQ1
-
 (2S)-3-sulfolactate + NADH + H+
-
 ?
(2S)-3-sulfolactaldehyde + NADP+ + H2O Pseudomonas putida
-
 (2S)-3-sulfolactate + NADPH + H+
-
 ?
(2S)-3-sulfolactaldehyde + NADP+ + H2O Pseudomonas putida SQ1
-
 (2S)-3-sulfolactate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida SQ1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-3-sulfolactaldehyde + NAD+ + H2O
-
 Pseudomonas putida (2S)-3-sulfolactate + NADH + H+
-
 ?
(2S)-3-sulfolactaldehyde + NAD+ + H2O
-
 Pseudomonas putida SQ1 (2S)-3-sulfolactate + NADH + H+
-
 ?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
-
 Pseudomonas putida (2S)-3-sulfolactate + NADPH + H+
-
 ?
(2S)-3-sulfolactaldehyde + NADP+ + H2O
-
 Pseudomonas putida SQ1 (2S)-3-sulfolactate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
SLA dehydrogenase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.4
-
 (2S)-3-sulfolactaldehyde with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
11.8
-
 (2S)-3-sulfolactaldehyde with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida
NADP+
-
 Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
81.4
-
 (2S)-3-sulfolactaldehyde with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
84.2
-
 (2S)-3-sulfolactaldehyde with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida