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Literature summary for 1.2.1.96 extracted from

  • Chen, Y.F.; Chao, H.; Zhou, N.Y.
    The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866 (2014), Appl. Microbiol. Biotechnol., 98, 1349-1356.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00357
-
4-hydroxybenzaldehyde with NADP+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.00636
-
4-hydroxy-3-methylbenzaldehyde with NADP+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.01311
-
4-hydroxybenzaldehyde with NAD+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.02055
-
4-hydroxy-3-methylbenzaldehyde with NAD+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzaldehyde + NADP+ + H2O Pseudomonas putida
-
4-hydroxybenzoic acid + NADPH + H+
-
?
4-hydroxybenzaldehyde + NADP+ + H2O Pseudomonas putida NCIMB 9866
-
4-hydroxybenzoic acid + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q59702
-
-
Pseudomonas putida NCIMB 9866 Q59702
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O low activity with NAD+ as cosubstrate Pseudomonas putida 4-hydroxy-3-methylbenzoic acid + NADH + H+
-
?
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O low activity with NAD+ as cosubstrate Pseudomonas putida NCIMB 9866 4-hydroxy-3-methylbenzoic acid + NADH + H+
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
-
Pseudomonas putida 4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
-
Pseudomonas putida NCIMB 9866 4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
?
4-hydroxybenzaldehyde + NAD+ + H2O low activity with NAD+ as cosubstrate Pseudomonas putida 4-hydroxybenzoic acid + NADH + H+
-
?
4-hydroxybenzaldehyde + NAD+ + H2O low activity with NAD+ as cosubstrate Pseudomonas putida NCIMB 9866 4-hydroxybenzoic acid + NADH + H+
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
-
Pseudomonas putida 4-hydroxybenzoic acid + NADPH + H+
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
-
Pseudomonas putida NCIMB 9866 4-hydroxybenzoic acid + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
p-hydroxybenzaldehyde dehydrogenase
-
Pseudomonas putida
PchA
-
Pseudomonas putida
PHBDD
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
4-hydroxybenzaldehyde with NAD+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.0017
-
4-hydroxy-3-methylbenzaldehyde with NAD+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.071
-
4-hydroxy-3-methylbenzaldehyde with NADP+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.26
-
4-hydroxybenzaldehyde with NADP+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.083
-
4-hydroxy-3-methylbenzaldehyde with NAD+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
0.13
-
4-hydroxybenzaldehyde with NAD+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
11.16
-
4-hydroxy-3-methylbenzaldehyde with NADP+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida
72.83
-
4-hydroxybenzaldehyde with NADP+ as cosubstrate, at pH 8.0 and 30°C Pseudomonas putida