Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00357 | - |
4-hydroxybenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.00636 | - |
4-hydroxy-3-methylbenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.01311 | - |
4-hydroxybenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.02055 | - |
4-hydroxy-3-methylbenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzaldehyde + NADP+ + H2O | Pseudomonas putida | - |
4-hydroxybenzoic acid + NADPH + H+ | - |
? | |
4-hydroxybenzaldehyde + NADP+ + H2O | Pseudomonas putida NCIMB 9866 | - |
4-hydroxybenzoic acid + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q59702 | - |
- |
Pseudomonas putida NCIMB 9866 | Q59702 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida | 4-hydroxy-3-methylbenzoic acid + NADH + H+ | - |
? | |
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida NCIMB 9866 | 4-hydroxy-3-methylbenzoic acid + NADH + H+ | - |
? | |
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida | 4-hydroxy-3-methylbenzoic acid + NADPH + H+ | - |
? | |
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida NCIMB 9866 | 4-hydroxy-3-methylbenzoic acid + NADPH + H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida | 4-hydroxybenzoic acid + NADH + H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida NCIMB 9866 | 4-hydroxybenzoic acid + NADH + H+ | - |
? | |
4-hydroxybenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida | 4-hydroxybenzoic acid + NADPH + H+ | - |
? | |
4-hydroxybenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida NCIMB 9866 | 4-hydroxybenzoic acid + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
p-hydroxybenzaldehyde dehydrogenase | - |
Pseudomonas putida |
PchA | - |
Pseudomonas putida |
PHBDD | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
4-hydroxybenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.0017 | - |
4-hydroxy-3-methylbenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.071 | - |
4-hydroxy-3-methylbenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.26 | - |
4-hydroxybenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
4-hydroxy-3-methylbenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
0.13 | - |
4-hydroxybenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
11.16 | - |
4-hydroxy-3-methylbenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
72.83 | - |
4-hydroxybenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida |