BRENDA - Enzyme Database
show all sequences of 1.2.1.96

The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866

Chen, Y.F.; Chao, H.; Zhou, N.Y.; Appl. Microbiol. Biotechnol. 98, 1349-1356 (2014)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00357
-
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.00636
-
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.01311
-
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.02055
-
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas putida
-
4-hydroxybenzoic acid + NADPH + H+
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas putida NCIMB 9866
-
4-hydroxybenzoic acid + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
Q59702
-
-
Pseudomonas putida NCIMB 9866
Q59702
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni2+-NTA column chromatography
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida
4-hydroxy-3-methylbenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida NCIMB 9866
4-hydroxy-3-methylbenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida
4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida NCIMB 9866
4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida
4-hydroxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida NCIMB 9866
4-hydroxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida NCIMB 9866
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
p-hydroxybenzaldehyde dehydrogenase
-
Pseudomonas putida
PchA
-
Pseudomonas putida
PHBDD
-
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0017
-
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.0017
-
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.071
-
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.26
-
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00357
-
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.00636
-
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.01311
-
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.02055
-
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas putida
-
4-hydroxybenzoic acid + NADPH + H+
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas putida NCIMB 9866
-
4-hydroxybenzoic acid + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA column chromatography
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida
4-hydroxy-3-methylbenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida NCIMB 9866
4-hydroxy-3-methylbenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida
4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida NCIMB 9866
4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida
4-hydroxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
low activity with NAD+ as cosubstrate
726788
Pseudomonas putida NCIMB 9866
4-hydroxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
-
726788
Pseudomonas putida NCIMB 9866
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0017
-
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.0017
-
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.071
-
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.26
-
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.083
-
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.13
-
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
11.16
-
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
72.83
-
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.083
-
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
0.13
-
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
11.16
-
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
72.83
-
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30C
Pseudomonas putida
Other publictions for EC 1.2.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741220
Mukherjee
Redirection of metabolite bios ...
Daucus carota
Planta
243
305-320
2016
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1
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726788
Chen
-
The catabolism of 2,4-xylenol ...
Pseudomonas putida, Pseudomonas putida NCIMB 9866
Appl. Microbiol. Biotechnol.
98
1349-1356
2014
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1
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4
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2
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2
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1
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8
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3
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4
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1
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1
1
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4
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2
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1
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8
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4
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4
288312
Cronin
Organization and sequences of ...
Pseudomonas putida
DNA Seq.
10
7-17
1999
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1
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1
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733948
Miller Jr
-
Bioconversion of toluene to p- ...
Pseudomonas putida, Pseudomonas putida EM2839
Green Chem.
1
143-152
1999
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2
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2
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2
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1
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1
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2
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2
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288314
Whited
Separation and partial charact ...
Pseudomonas mendocina, Pseudomonas mendocina KR1
J. Bacteriol.
173
3017-3020
1991
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2
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2
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1
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1
1
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2
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1
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1
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2
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1
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1
1
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2
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