Cloned (Comment) | Organism |
---|---|
into the pET29 vector for expression in Escherichia coli BL21DE3 cells | Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
A(plusCTE) | chimeric mutant for testing the regulatory function of CTE | Spinacia oleracea |
B(E326Q) | site specific mutant of the GAPDH B-subunit | Spinacia oleracea |
B(minCTE) | deletion mutant for testing the regulatory function of CTE | Spinacia oleracea |
B(R77A) | site specific mutant of the GAPDH B-subunit | Spinacia oleracea |
B(S188A) | site specific mutant of the GAPDH B-subunit | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
3-phospho-D-glyceroyl phosphate | mutant B(E362Q) | Spinacia oleracea | |
0.015 | - |
3-phospho-D-glyceroyl phosphate | GapA | Spinacia oleracea | |
0.015 | - |
3-phospho-D-glyceroyl phosphate | GapB, analyzed under reducing conditions | Spinacia oleracea | |
0.016 | - |
3-phospho-D-glyceroyl phosphate | mutant B(R77A) | Spinacia oleracea | |
0.019 | - |
3-phospho-D-glyceroyl phosphate | A(plusCTE) mutant, analyzed under oxidizing conditions | Spinacia oleracea | |
0.019 | - |
NADPH | mutant B(minCTE) | Spinacia oleracea | |
0.02 | - |
3-phospho-D-glyceroyl phosphate | AB-GAPDH, analyzed under reducing conditions | Spinacia oleracea | |
0.02 | - |
3-phospho-D-glyceroyl phosphate | GapB, analyzed under oxidizing conditions | Spinacia oleracea | |
0.021 | - |
3-phospho-D-glyceroyl phosphate | A(plusCTE) mutant, analyzed under reducing conditions | Spinacia oleracea | |
0.022 | - |
3-phospho-D-glyceroyl phosphate | B(minCTE) mutant | Spinacia oleracea | |
0.024 | - |
3-phospho-D-glyceroyl phosphate | AB-GAPDH, analyzed under oxidizing conditions | Spinacia oleracea | |
0.024 | - |
NADPH | mutant B(188)A, analyzed under oxidizing conditions | Spinacia oleracea | |
0.024 | - |
NADPH | mutant B(E362Q) | Spinacia oleracea | |
0.027 | - |
3-phospho-D-glyceroyl phosphate | mutant B(188)A, analyzed under oxidizing conditions | Spinacia oleracea | |
0.029 | - |
NADPH | GapA | Spinacia oleracea | |
0.03 | - |
NADPH | GapB, analyzed under reducing conditions | Spinacia oleracea | |
0.031 | - |
3-phospho-D-glyceroyl phosphate | mutant B(188)A, analyzed under reducing conditions | Spinacia oleracea | |
0.038 | - |
NADPH | GapB, analyzed under oxidizing conditions | Spinacia oleracea | |
0.043 | - |
NADPH | A(plusCTE) mutant, analyzed under oxidizing conditions | Spinacia oleracea | |
0.046 | - |
NADPH | mutant B(188)A, analyzed under reducing conditions | Spinacia oleracea | |
0.05 | - |
NADPH | AB-GAPDH, analyzed under oxidizing conditions | Spinacia oleracea | |
0.05 | - |
NADPH | AB-GAPDH, analyzed under reducing conditions | Spinacia oleracea | |
0.065 | - |
NADPH | A(plusCTE) mutant, analyzed under reducing conditions | Spinacia oleracea | |
0.078 | - |
NADPH | mutant B(R77A) | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
132000 | - |
GapA, estimated by gel filtration | Spinacia oleracea |
151000 | - |
B(minCTE) mutant, estimated by gel filtration | Spinacia oleracea |
189000 | - |
tetrameric conformation of GAPDH-A2B2 stabilized by NADPH, estimated by gel filtration | Spinacia oleracea |
232000 | - |
GapB mutant B(E326Q) in the presence of NADPH, estimated by gel filtration | Spinacia oleracea |
243000 | - |
wild-type GapB, tetramer in the presence of NADPH, estimated by gel filtration | Spinacia oleracea |
247000 | - |
GapB mutant B(S188A) in the presence of NADPH, estimated by gel filtration | Spinacia oleracea |
270000 | - |
A(plusCTE) mutant, tetramer in the presence of NADPH, estimated by gel filtration | Spinacia oleracea |
278000 | - |
GapB mutant B(R77A) in the presence of NADPH, estimated by gel filtration | Spinacia oleracea |
491000 | - |
wild-type GapB in the presence of NADH, compatible with octameric structure, estimated by gel filtration | Spinacia oleracea |
520000 | - |
GapB mutant B(E326Q) in the presence of NADH, compatible with octameric structure, estimated by gel filtration | Spinacia oleracea |
520000 | - |
GapB mutant B(R77A) in the presence of NADH, compatible with octameric structure, estimated by gel filtration | Spinacia oleracea |
553000 | - |
GapB mutant B(S188A) in the presence of NADH, compatible with octameric structure, estimated by gel filtration | Spinacia oleracea |
760000 | - |
oligomer of GAPDH in the presence of NADH, suggesting A8B8 stoichiometry, estimated by gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADPH + H+ | Spinacia oleracea | - |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Q-Sepharose HP and a MonoQ anion-exchange column | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
Spinacia oleracea | D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Spinacia oleracea |
glyceraldehyde-3-phosphate dehydrogenase | - |
Spinacia oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
activity assay | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Spinacia oleracea |