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show all sequences of 1.2.1.89

Refolding of a thermostable glyceraldehyde dehydrogenase for application in synthetic cascade biomanufacturing

Steffler, F.; Sieber, V.; PLoS One 8, e70592 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the enzyme accept NAD+ as cofactor under technically relevant conditions, making it suitable for application in the artificial glycolysis by synthetic cascade biomanufacturing
Thermoplasma acidophilum
Cloned(Commentary)
Commentary
Organism
the gene of TaAlDH is synthesized and cloned into a T7 based vector system for recombinant expression in Escherichia coli. Using matrix-assisted refolding of inclusion bodies the yield of enzyme production is enhanced 43fold
Thermoplasma acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
NADP+
pH 6.2, 50°C
Thermoplasma acidophilum
Organic Solvent Stability
Organic Solvent
Commentary
Organism
guanidine-HCl
the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C
Thermoplasma acidophilum
isobutanol
the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C
Thermoplasma acidophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermoplasma acidophilum
Q9HK01
-
-
Thermoplasma acidophilum JCM 9062
Q9HK01
-
-
Purification (Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADP+ + H2O
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM
726348
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM
726348
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
additional information
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
726348
Thermoplasma acidophilum
?
-
-
-
-
additional information
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
726348
Thermoplasma acidophilum JCM 9062
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
assay at
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM, Km for NADP+: 0.022 mM, Km for NAD+ is 22.13 mM, at H 6.2, 50°C
Thermoplasma acidophilum
Application (protein specific)
Application
Commentary
Organism
synthesis
the enzyme accept NAD+ as cofactor under technically relevant conditions, making it suitable for application in the artificial glycolysis by synthetic cascade biomanufacturing
Thermoplasma acidophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
the gene of TaAlDH is synthesized and cloned into a T7 based vector system for recombinant expression in Escherichia coli. Using matrix-assisted refolding of inclusion bodies the yield of enzyme production is enhanced 43fold
Thermoplasma acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM, Km for NADP+: 0.022 mM, Km for NAD+ is 22.13 mM, at H 6.2, 50°C
Thermoplasma acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
NADP+
pH 6.2, 50°C
Thermoplasma acidophilum
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
guanidine-HCl
the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C
Thermoplasma acidophilum
isobutanol
the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C
Thermoplasma acidophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADP+ + H2O
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM
726348
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
also accepts NAD+ under technically relevant conditions, cofactor above 1 mM
726348
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
additional information
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
726348
Thermoplasma acidophilum
?
-
-
-
-
additional information
no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose
726348
Thermoplasma acidophilum JCM 9062
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
assay at
Thermoplasma acidophilum
Other publictions for EC 1.2.1.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740187
Wu
A new NAD(+)-dependent glycera ...
Escherichia coli
Biosci. Biotechnol. Biochem.
80
2306-2310
2016
-
-
-
-
3
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
3
-
1
-
-
-
1
3
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
3
3
739818
Iermak
-
Crystallization behaviour of g ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
71
1475-1480
2015
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726348
Steffler
Refolding of a thermostable gl ...
Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
PLoS One
8
e70592
2013
-
1
1
-
-
-
-
1
-
-
-
-
2
6
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
736023
Steffler
Improvement of thermostable al ...
Thermoplasma acidophilum
Enzyme Microb. Technol.
53
307-314
2013
-
-
1
-
13
-
-
6
-
-
1
-
1
1
-
-
1
-
-
-
-
-
4
1
-
-
-
3
-
-
-
3
-
-
-
-
-
1
3
-
13
-
-
-
-
6
-
-
1
-
1
-
-
1
-
-
-
-
4
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
724216
Jung
Identification and characteriz ...
Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
Biochem. J.
397
131-138
2006
5
-
-
-
-
-
8
2
-
-
3
2
-
8
-
-
1
-
-
-
1
-
10
2
1
1
2
-
1
1
1
1
-
-
-
5
-
-
1
-
-
-
-
8
-
2
-
-
3
2
-
-
-
1
-
-
1
-
10
2
1
1
2
-
1
1
1
-
-
1
1
-
-
-
724992
Reher
Glyceraldehyde dehydrogenases ...
Picrophilus torridus, Picrophilus torridus DSM 9790, Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
FEBS Lett.
580
1198-1204
2006
-
-
1
-
-
-
1
8
-
-
6
4
-
9
-
-
2
-
-
-
1
-
18
2
4
-
4
8
4
2
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
8
-
-
6
4
-
-
-
2
-
-
1
-
18
2
4
-
4
8
4
2
-
-
-
2
2
-
8
8