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show all sequences of 1.2.1.89

Glyceraldehyde dehydrogenases from the thermoacidophilic euryarchaeota Picrophilus torridus and Thermoplasma acidophilum, key enzymes of the non-phosphorylative Entner-Doudoroff pathway, constitute a novel enzyme family within the aldehyde dehydrogenase superfamily

Reher, M.; Schönheit, P.; FEBS Lett. 580, 1198-1204 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Picrophilus torridus
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glyceraldehyde
slight substrate inhibition, 25% inhibition at 2 mM
Picrophilus torridus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
D-glyceraldehyde
pH 6.9, 58°C
Picrophilus torridus
0.2
-
D-glyceraldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
0.36
-
NADP+
pH 6.9, 58°C
Thermoplasma acidophilum
0.42
-
NADP+
pH 6.9, 58°C
Picrophilus torridus
5.1
-
D-glycerate
pH 6.9, 58°C
Picrophilus torridus
11.1
-
glycolaldehyde
pH 6.9, 58°C
Picrophilus torridus
12.3
-
glycolaldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
18.3
-
D-glycerate
pH 6.9, 58°C
Thermoplasma acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54700
-
2 * 54700, calculated from sequence
Thermoplasma acidophilum
55200
-
2 * 55200, calculated from sequence
Picrophilus torridus
56000
-
2 * 56000, SDS-PAGE
Picrophilus torridus
56000
-
2 * 56000, SDS-PAGE
Thermoplasma acidophilum
115000
-
gel filtration
Picrophilus torridus
120000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Picrophilus torridus
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum JCM 9062
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Picrophilus torridus DSM 9790
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Picrophilus torridus
Q6L285
-
-
Picrophilus torridus DSM 9790
Q6L285
-
-
Thermoplasma acidophilum
Q9HK01
-
-
Thermoplasma acidophilum JCM 9062
Q9HK01
-
-
Purification (Commentary)
Commentary
Organism
-
Picrophilus torridus
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6
-
pH 6.9, 58°C
Picrophilus torridus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Picrophilus torridus
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Picrophilus torridus
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Picrophilus torridus DSM 9790
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Picrophilus torridus DSM 9790
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum JCM 9062
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus DSM 9790
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum JCM 9062
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus DSM 9790
glycolate + NADPH + H+
-
-
-
?
additional information
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
724992
Picrophilus torridus
?
-
-
-
-
additional information
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
724992
Picrophilus torridus DSM 9790
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 55200, calculated from sequence; 2 * 56000, SDS-PAGE
Picrophilus torridus
homodimer
2 * 54700, calculated from sequence; 2 * 56000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
58
-
assay at
Picrophilus torridus
58
-
assay at
Thermoplasma acidophilum
63
-
-
Picrophilus torridus
63
-
-
Thermoplasma acidophilum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
2 h, stable
Picrophilus torridus
60
-
2 h, stable
Thermoplasma acidophilum
70
-
half-life: 15 min
Picrophilus torridus
70
-
half-life: 40 min
Thermoplasma acidophilum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10
-
D-glyceraldehyde
pH 6.9, 58°C
Picrophilus torridus
10
-
NADP+
pH 6.9, 58°C
Picrophilus torridus
12
-
D-glycerate
pH 6.9, 58°C
Picrophilus torridus
20
-
D-glycerate
pH 6.9, 58°C
Thermoplasma acidophilum
20
-
glycolaldehyde
pH 6.9, 58°C
Picrophilus torridus
25
-
NADP+
pH 6.9, 58°C
Thermoplasma acidophilum
26
-
D-glyceraldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
37
-
glycolaldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Picrophilus torridus
6.9
-
assay at
Picrophilus torridus
7.6
-
-
Thermoplasma acidophilum
7.9
-
assay at
Thermoplasma acidophilum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.3
8.1
pH 4.3: about 50% of maximal activity, pH 8.1: about 50% of maximal activity
Picrophilus torridus
5.8
8.7
pH 5.8: about 50% of maximal activity, pH 8.7: about 50% of maximal activity
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
completely inactive with NAD+
Picrophilus torridus
NADP+
completely inactive with NAD+
Thermoplasma acidophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Picrophilus torridus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
completely inactive with NAD+
Picrophilus torridus
NADP+
completely inactive with NAD+
Thermoplasma acidophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glyceraldehyde
slight substrate inhibition, 25% inhibition at 2 mM
Picrophilus torridus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
D-glyceraldehyde
pH 6.9, 58°C
Picrophilus torridus
0.2
-
D-glyceraldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
0.36
-
NADP+
pH 6.9, 58°C
Thermoplasma acidophilum
0.42
-
NADP+
pH 6.9, 58°C
Picrophilus torridus
5.1
-
D-glycerate
pH 6.9, 58°C
Picrophilus torridus
11.1
-
glycolaldehyde
pH 6.9, 58°C
Picrophilus torridus
12.3
-
glycolaldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
18.3
-
D-glycerate
pH 6.9, 58°C
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54700
-
2 * 54700, calculated from sequence
Thermoplasma acidophilum
55200
-
2 * 55200, calculated from sequence
Picrophilus torridus
56000
-
2 * 56000, SDS-PAGE
Picrophilus torridus
56000
-
2 * 56000, SDS-PAGE
Thermoplasma acidophilum
115000
-
gel filtration
Picrophilus torridus
120000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Picrophilus torridus
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum JCM 9062
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Picrophilus torridus DSM 9790
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Picrophilus torridus
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6
-
pH 6.9, 58°C
Picrophilus torridus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Picrophilus torridus
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Picrophilus torridus
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
724992
Picrophilus torridus DSM 9790
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive with NAD+
724992
Picrophilus torridus DSM 9790
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for glyceraldehyde 3-phosphate is less than 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum JCM 9062
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
catalytic efficiency (kcat/Km) for D-glyceraldehyde 3-phosphate is 1.4% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus DSM 9790
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is about 2% compared to the catalytic efficiency with D-glyceraldehyde
724992
Thermoplasma acidophilum JCM 9062
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
catalytic efficiency (kcat/Km) for glycolaldehyde is 1% compared to the catalytic efficiency with D-glyceraldehyde
724992
Picrophilus torridus DSM 9790
glycolate + NADPH + H+
-
-
-
?
additional information
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
724992
Picrophilus torridus
?
-
-
-
-
additional information
no activity with: acetaldehyde, crotonaldehyde, benzaldehyde, succinate semialdehyde, and dihydroxyacetone
724992
Picrophilus torridus DSM 9790
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 55200, calculated from sequence; 2 * 56000, SDS-PAGE
Picrophilus torridus
homodimer
2 * 54700, calculated from sequence; 2 * 56000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
58
-
assay at
Picrophilus torridus
58
-
assay at
Thermoplasma acidophilum
63
-
-
Picrophilus torridus
63
-
-
Thermoplasma acidophilum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
2 h, stable
Picrophilus torridus
60
-
2 h, stable
Thermoplasma acidophilum
70
-
half-life: 15 min
Picrophilus torridus
70
-
half-life: 40 min
Thermoplasma acidophilum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10
-
D-glyceraldehyde
pH 6.9, 58°C
Picrophilus torridus
10
-
NADP+
pH 6.9, 58°C
Picrophilus torridus
12
-
D-glycerate
pH 6.9, 58°C
Picrophilus torridus
20
-
D-glycerate
pH 6.9, 58°C
Thermoplasma acidophilum
20
-
glycolaldehyde
pH 6.9, 58°C
Picrophilus torridus
25
-
NADP+
pH 6.9, 58°C
Thermoplasma acidophilum
26
-
D-glyceraldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
37
-
glycolaldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Picrophilus torridus
6.9
-
assay at
Picrophilus torridus
7.6
-
-
Thermoplasma acidophilum
7.9
-
assay at
Thermoplasma acidophilum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.3
8.1
pH 4.3: about 50% of maximal activity, pH 8.1: about 50% of maximal activity
Picrophilus torridus
5.8
8.7
pH 5.8: about 50% of maximal activity, pH 8.7: about 50% of maximal activity
Thermoplasma acidophilum
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
Picrophilus torridus
physiological function
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
Thermoplasma acidophilum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
Picrophilus torridus
physiological function
the enzyme is involved in the non-phosphorylative Entner–Doudoroff pathway
Thermoplasma acidophilum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1
-
D-glycerate
pH 6.9, 58°C
Thermoplasma acidophilum
1.8
-
glycolaldehyde
pH 6.9, 58°C
Picrophilus torridus
2.4
-
D-glycerate
pH 6.9, 58°C
Picrophilus torridus
3
-
glycolaldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
24
-
NADP+
pH 6.9, 58°C
Picrophilus torridus
70
-
NADP+
pH 6.9, 58°C
Thermoplasma acidophilum
130
-
D-glyceraldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
167
-
D-glyceraldehyde
pH 6.9, 58°C
Picrophilus torridus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1
-
D-glycerate
pH 6.9, 58°C
Thermoplasma acidophilum
1.8
-
glycolaldehyde
pH 6.9, 58°C
Picrophilus torridus
2.4
-
D-glycerate
pH 6.9, 58°C
Picrophilus torridus
3
-
glycolaldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
24
-
NADP+
pH 6.9, 58°C
Picrophilus torridus
70
-
NADP+
pH 6.9, 58°C
Thermoplasma acidophilum
130
-
D-glyceraldehyde
pH 6.9, 58°C
Thermoplasma acidophilum
167
-
D-glyceraldehyde
pH 6.9, 58°C
Picrophilus torridus
Other publictions for EC 1.2.1.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740187
Wu
A new NAD(+)-dependent glycera ...
Escherichia coli
Biosci. Biotechnol. Biochem.
80
2306-2310
2016
-
-
-
-
3
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
3
-
1
-
-
-
1
3
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
3
3
739818
Iermak
-
Crystallization behaviour of g ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
71
1475-1480
2015
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726348
Steffler
Refolding of a thermostable gl ...
Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
PLoS One
8
e70592
2013
-
1
1
-
-
-
-
1
-
-
-
-
2
6
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
736023
Steffler
Improvement of thermostable al ...
Thermoplasma acidophilum
Enzyme Microb. Technol.
53
307-314
2013
-
-
1
-
13
-
-
6
-
-
1
-
1
1
-
-
1
-
-
-
-
-
4
1
-
-
-
3
-
-
-
3
-
-
-
-
-
1
3
-
13
-
-
-
-
6
-
-
1
-
1
-
-
1
-
-
-
-
4
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
724216
Jung
Identification and characteriz ...
Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
Biochem. J.
397
131-138
2006
5
-
-
-
-
-
8
2
-
-
3
2
-
8
-
-
1
-
-
-
1
-
10
2
1
1
2
-
1
1
1
1
-
-
-
5
-
-
1
-
-
-
-
8
-
2
-
-
3
2
-
-
-
1
-
-
1
-
10
2
1
1
2
-
1
1
1
-
-
1
1
-
-
-
724992
Reher
Glyceraldehyde dehydrogenases ...
Picrophilus torridus, Picrophilus torridus DSM 9790, Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
FEBS Lett.
580
1198-1204
2006
-
-
1
-
-
-
1
8
-
-
6
4
-
9
-
-
2
-
-
-
1
-
18
2
4
-
4
8
4
2
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
8
-
-
6
4
-
-
-
2
-
-
1
-
18
2
4
-
4
8
4
2
-
-
-
2
2
-
8
8