BRENDA - Enzyme Database show
show all sequences of 1.2.1.89

Identification and characterization of Thermoplasma acidophilum glyceraldehyde dehydrogenase: a new class of NADP+-specific aldehyde dehydrogenase

Jung, J.H.; Lee, S.B.; Biochem. J. 397, 131-138 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, activity is enhanced more than 2fold
Thermoplasma acidophilum
4-chloromercuribenzoic acid
1 mM, completely inhibits activity
Thermoplasma acidophilum
4-hydroxymercuribenzoic acid
1 mM, completely inhibits activity
Thermoplasma acidophilum
DTT
1 mM, activity is enhanced more than 2fold
Thermoplasma acidophilum
glutathione
1 mM, activity is enhanced more than 2fold
Thermoplasma acidophilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, 12 h, 4°C, complete loss of activity
Thermoplasma acidophilum
Cd2+
1 mM, 12 h, 4°C, 93% loss of activity
Thermoplasma acidophilum
Cu2+
1 mM, 12 h, 4°C, complete loss of activity
Thermoplasma acidophilum
Hg2+
1 mM, 12 h, 4°C, 99% loss of activity
Thermoplasma acidophilum
Mg2+
1 mM, 12 h, 4°C, 24% loss of activity
Thermoplasma acidophilum
Mn2+
1 mM, 12 h, 4°C, 44% loss of activity
Thermoplasma acidophilum
Ni2+
1 mM, 12 h, 4°C, 58% loss of activity
Thermoplasma acidophilum
Zn2+
1 mM, 12 h, 4°C, 46% loss of activity
Thermoplasma acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
NADP+
pH 8.0, 50°C
Thermoplasma acidophilum
0.33
-
D-glyceraldehyde
pH 8.0, 50°C
Thermoplasma acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
4 * 53000, SDS-PAGE
Thermoplasma acidophilum
54782
-
4 * 54782, calculated from sequence
Thermoplasma acidophilum
215000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum JCM 9062
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermoplasma acidophilum
Q9HK01
-
-
Thermoplasma acidophilum JCM 9062
Q9HK01
-
-
Purification (Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.85
-
pH 8.0, 50°C
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
724216
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive when NAD+ is substituted for NADP+
724216
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
724216
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive when NAD+ is substituted for NADP+
724216
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
activity is 33% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
activity is 33% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum JCM 9062
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
activity is 14% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
activity is 14% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum JCM 9062
glycolate + NADPH + H+
-
-
-
?
additional information
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
724216
Thermoplasma acidophilum
?
-
-
-
-
additional information
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
724216
Thermoplasma acidophilum JCM 9062
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 53000, SDS-PAGE
Thermoplasma acidophilum
tetramer
4 * 54782, calculated from sequence
Thermoplasma acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
55
-
Thermoplasma acidophilum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
70
30°C: about 50% of maximal activity, 70°C: about 60% of maximal activity
Thermoplasma acidophilum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
stable for 2 h
Thermoplasma acidophilum
80
-
activity is decreased to less than 50% within 20 min
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Thermoplasma acidophilum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 65% of maximal activity, pH 10.0: about 70% of maximal activity
Thermoplasma acidophilum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
24 h, stable
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
completely inactive when NAD+ is substituted for NADP+
Thermoplasma acidophilum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, activity is enhanced more than 2fold
Thermoplasma acidophilum
4-chloromercuribenzoic acid
1 mM, completely inhibits activity
Thermoplasma acidophilum
4-hydroxymercuribenzoic acid
1 mM, completely inhibits activity
Thermoplasma acidophilum
DTT
1 mM, activity is enhanced more than 2fold
Thermoplasma acidophilum
glutathione
1 mM, activity is enhanced more than 2fold
Thermoplasma acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
completely inactive when NAD+ is substituted for NADP+
Thermoplasma acidophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, 12 h, 4°C, complete loss of activity
Thermoplasma acidophilum
Cd2+
1 mM, 12 h, 4°C, 93% loss of activity
Thermoplasma acidophilum
Cu2+
1 mM, 12 h, 4°C, complete loss of activity
Thermoplasma acidophilum
Hg2+
1 mM, 12 h, 4°C, 99% loss of activity
Thermoplasma acidophilum
Mg2+
1 mM, 12 h, 4°C, 24% loss of activity
Thermoplasma acidophilum
Mn2+
1 mM, 12 h, 4°C, 44% loss of activity
Thermoplasma acidophilum
Ni2+
1 mM, 12 h, 4°C, 58% loss of activity
Thermoplasma acidophilum
Zn2+
1 mM, 12 h, 4°C, 46% loss of activity
Thermoplasma acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
NADP+
pH 8.0, 50°C
Thermoplasma acidophilum
0.33
-
D-glyceraldehyde
pH 8.0, 50°C
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
4 * 53000, SDS-PAGE
Thermoplasma acidophilum
54782
-
4 * 54782, calculated from sequence
Thermoplasma acidophilum
215000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
D-glyceraldehyde + NADP+ + H2O
Thermoplasma acidophilum JCM 9062
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
D-glycerate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.85
-
pH 8.0, 50°C
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
724216
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive when NAD+ is substituted for NADP+
724216
Thermoplasma acidophilum
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
724216
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde + NADP+ + H2O
completely inactive when NAD+ is substituted for NADP+
724216
Thermoplasma acidophilum JCM 9062
D-glycerate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
activity is 33% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
activity is 33% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum JCM 9062
D-glycerate 3-phosphate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
activity is 14% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum
glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+
activity is 14% compared to the activity with D-glyceraldehyde
724216
Thermoplasma acidophilum JCM 9062
glycolate + NADPH + H+
-
-
-
?
additional information
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
724216
Thermoplasma acidophilum
?
-
-
-
-
additional information
no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde)
724216
Thermoplasma acidophilum JCM 9062
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 53000, SDS-PAGE
Thermoplasma acidophilum
tetramer
4 * 54782, calculated from sequence
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
55
-
Thermoplasma acidophilum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
70
30°C: about 50% of maximal activity, 70°C: about 60% of maximal activity
Thermoplasma acidophilum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
stable for 2 h
Thermoplasma acidophilum
80
-
activity is decreased to less than 50% within 20 min
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Thermoplasma acidophilum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 65% of maximal activity, pH 10.0: about 70% of maximal activity
Thermoplasma acidophilum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
24 h, stable
Thermoplasma acidophilum
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
Thermoplasma acidophilum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway
Thermoplasma acidophilum
Other publictions for EC 1.2.1.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740187
Wu
A new NAD(+)-dependent glycera ...
Escherichia coli
Biosci. Biotechnol. Biochem.
80
2306-2310
2016
-
-
-
-
3
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
3
-
1
-
-
-
1
3
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
3
3
739818
Iermak
-
Crystallization behaviour of g ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
71
1475-1480
2015
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726348
Steffler
Refolding of a thermostable gl ...
Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
PLoS One
8
e70592
2013
-
1
1
-
-
-
-
1
-
-
-
-
2
6
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
736023
Steffler
Improvement of thermostable al ...
Thermoplasma acidophilum
Enzyme Microb. Technol.
53
307-314
2013
-
-
1
-
13
-
-
6
-
-
1
-
1
1
-
-
1
-
-
-
-
-
4
1
-
-
-
3
-
-
-
3
-
-
-
-
-
1
3
-
13
-
-
-
-
6
-
-
1
-
1
-
-
1
-
-
-
-
4
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
724216
Jung
Identification and characteriz ...
Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
Biochem. J.
397
131-138
2006
5
-
-
-
-
-
8
2
-
-
3
2
-
8
-
-
1
-
-
-
1
-
10
2
1
1
2
-
1
1
1
1
-
-
-
5
-
-
1
-
-
-
-
8
-
2
-
-
3
2
-
-
-
1
-
-
1
-
10
2
1
1
2
-
1
1
1
-
-
1
1
-
-
-
724992
Reher
Glyceraldehyde dehydrogenases ...
Picrophilus torridus, Picrophilus torridus DSM 9790, Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062
FEBS Lett.
580
1198-1204
2006
-
-
1
-
-
-
1
8
-
-
6
4
-
9
-
-
2
-
-
-
1
-
18
2
4
-
4
8
4
2
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
8
-
-
6
4
-
-
-
2
-
-
1
-
18
2
4
-
4
8
4
2
-
-
-
2
2
-
8
8