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Literature summary for 1.2.1.88 extracted from
- Enzymes of DELTA1-pyrroline-5-carboxylate metabolism in the camel tick Hyalomma dromedarii during embryogenesis. Purification and characterization of DELTA1-pyrroline-5-carboxylate dehydrogenases (1997), Comp. Biochem. Physiol. B, 118, 229-237.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminobutyrate | both isoforms | Hyalomma dromedarii |  |
| ADP | 3 mM, 14% inhibition of isoform A and 16% inhibition of isoform B | Hyalomma dromedarii | |
| AMP | 3 mM, 33% inhibition of isoform A and 11% inhibition of isoform B | Hyalomma dromedarii | |
| arsenite | both isoforms | Hyalomma dromedarii | |
| ATP | inhibits isoform B by 33%, but not isoform A | Hyalomma dromedarii | |
| Co2+ | both isoforms, concentration above 3 mM | Hyalomma dromedarii | |
| Gly | - |
Hyalomma dromedarii | |
| Hg2+ | both isoforms | Hyalomma dromedarii | |
| iodoacetamide | both isoforms | Hyalomma dromedarii | |
| L-Ala | - |
Hyalomma dromedarii | |
| L-Glu | - |
Hyalomma dromedarii | |
| L-hydroxyproline | both isoforms, potent inhibition | Hyalomma dromedarii | |
| L-Leu | - |
Hyalomma dromedarii | |
| L-Ser | - |
Hyalomma dromedarii | |
| L-Val | - |
Hyalomma dromedarii | |
| Ni2+ | both isoforms | Hyalomma dromedarii | |
| p-hydroxymercuribenzoate | both isoforms, potent inhibition | Hyalomma dromedarii | |
| proline | both isoforms | Hyalomma dromedarii | |
| Zn2+ | both isoforms | Hyalomma dromedarii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.133 | - |
D-glucose | isoform A | Hyalomma dromedarii | |
| 0.2 | - |
1-pyrroline-5-carboxylate | isoform B, with NADP+ | Hyalomma dromedarii | |
| 0.2 | - |
indol-3-acetaldehyde | isoform A | Hyalomma dromedarii | |
| 0.25 | - |
4-methoxybenzaldehyde | isoform A | Hyalomma dromedarii | |
| 0.27 | - |
p-nitrobenzaldehyde | isoform A | Hyalomma dromedarii | |
| 0.3 | - |
1-pyrroline-5-carboxylate | isoform B, with NAD+ | Hyalomma dromedarii | |
| 0.3 | - |
1-pyrroline-5-carboxylate | isoform A, with NADP+ | Hyalomma dromedarii | |
| 0.33 | - |
1-pyrroline-5-carboxylate | isoform A, with NAD+ | Hyalomma dromedarii | |
| 0.5 | - |
benzaldehyde | isoform A | Hyalomma dromedarii | |
| 0.5 | - |
3-Methoxybenzaldehyde | isoform A | Hyalomma dromedarii | |
| 0.62 | - |
NAD+ | isoform A | Hyalomma dromedarii | |
| 0.76 | - |
formaldehyde | isoform A | Hyalomma dromedarii | |
| 1 | - |
NADP+ | isoform A | Hyalomma dromedarii | |
| 1 | - |
acetaldehyde | isoform A | Hyalomma dromedarii | |
| 1 | - |
glyoxylic acid | isoform A | Hyalomma dromedarii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
isoform B, gel filtration | Hyalomma dromedarii |
| 52000 | - |
1 * 52000, isoform B, SDS-PAGE | Hyalomma dromedarii |
| 59000 | - |
1 * 59000, isoform A, SDS-PAGE | Hyalomma dromedarii |
| 60000 | - |
isoform A, gel filtration | Hyalomma dromedarii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyalomma dromedarii | - |
two isoforms A and B | - |
| Hyalomma dromedarii | - |
camel tick | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amino acid composition determination | Hyalomma dromedarii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Hyalomma dromedarii | - |
| larva | - |
Hyalomma dromedarii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.151 | - |
embryo, 24 days after oviposition | Hyalomma dromedarii |
| 0.223 | - |
larvae | Hyalomma dromedarii |
| 9.75 | - |
purified enzyme | Hyalomma dromedarii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Hyalomma dromedarii | L-glutamate + NADH | - |
? | |
| 1-pyrroline-5-carboxylate + NADP+ + H2O | - |
Hyalomma dromedarii | L-glutamate + NADPH + H+ | - |
r | |
| 3-methoxybenzaldehyde + NAD+ + H2O | as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B | Hyalomma dromedarii | 3-methoxybenzoate + NADH | - |
? | |
| 4-methoxybenzaldehyde + NAD+ + H2O | as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B | Hyalomma dromedarii | 4-methoxybenzoate + NADH + H+ | - |
? | |
| 4-nitrobenzaldehyde + NAD+ + H2O | as active as 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B | Hyalomma dromedarii | 4-nitrobenzoate + NADH + H+ | - |
? | |
| acetaldehyde + NAD+ + H2O | 41% activity compared to 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B | Hyalomma dromedarii | acetate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | 2fold higher activity than with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B | Hyalomma dromedarii | benzoate + NADH + H+ | - |
? | |
| D-glucose + NAD+ + H2O | 80% of the activity with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B | Hyalomma dromedarii | D-gluconate + NADH | - |
? | |
| formaldehyde + NAD+ + H2O | 50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B | Hyalomma dromedarii | formate + NADH | - |
? | |
| glyceralaldehyde-3-phosphate + NAD+ + H2O | slight activity with isoforms A and B | Hyalomma dromedarii | glycerate-3-phosphate + NADH | - |
? | |
| glyoxylic acid + NAD+ + H2O | 41% activity of that with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B | Hyalomma dromedarii | oxalic acid + NADH | - |
? | |
| indole-3-acetaldehyde + NAD+ + H2O | 66% activity of that with 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B | Hyalomma dromedarii | indole-3-acetate + NADH + H+ | - |
? | |
| indole-3-phosphate + NAD+ + H2O | slight activity with isoforms A and B | Hyalomma dromedarii | ? | - |
? | |
| additional information | additional esterase activity with p-nitrophenyl acetate as substrate, 50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B | Hyalomma dromedarii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 59000, isoform A, SDS-PAGE | Hyalomma dromedarii |
| monomer | 1 * 52000, isoform B, SDS-PAGE | Hyalomma dromedarii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P5CD | - |
Hyalomma dromedarii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
both isoforms | Hyalomma dromedarii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
proline | isoform B | Hyalomma dromedarii | |
| 3 | - |
gamma-aminobutyrate | isoform B | Hyalomma dromedarii | |
| 3 | - |
proline | isoform A | Hyalomma dromedarii | |
| 3.4 | - |
glutamate | isoform B | Hyalomma dromedarii | |
| 3.75 | - |
hydroxyproline | isoform B | Hyalomma dromedarii | |
| 3.75 | - |
gamma-aminobutyrate | isoform A | Hyalomma dromedarii | |
| 4.75 | - |
hydroxyproline | isoform A | Hyalomma dromedarii | |
| 7 | - |
glutamate | isoform A | Hyalomma dromedarii | |
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Release 2023.1 (January 2023)
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