Literature summary for 1.2.1.8 extracted from

Halavaty, A.S.; Rich, R.L.; Chen, C.; Joo, J.C.; Minasov, G.; Dubrovska, I.; Winsor, J.R.; Myszka, D.G.; Duban, M.; Shuvalova, L.; Yakunin, A.F.; Anderson, W.F.
Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus (2015), Acta Crystallogr. Sect. D, 71, 1159-1175.

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Activating Compound

Activating Compound	Comment	Organism	Structure
KCl	maximum activity at 0.5 M	Staphylococcus aureus
NaCl	maximum activity at 0.5 M	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)/pMAGIC cells	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 200 mM MgCl2,100 mM Tris pH 8.5, 20% (w/v) PEG 8000	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
G234S	the mutant shows increased activity and reduced substrate inhibition compared to the wild type enzyme	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
Betaine aldehyde	strongly inhibited by betaine aldehyde at concentrations of more than 0.15 mM	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.17	-	Betaine aldehyde	at pH 8.0 and 30°C	Staphylococcus aureus
0.43	-	NAD+	at pH 8.0 and 30°C	Staphylococcus aureus
2.26	-	NADP+	at pH 8.0 and 30°C	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	4 * 60000, SDS-PAGE	Staphylococcus aureus
215000	-	gel filtration	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
betaine aldehyde + NAD+ + H2O	Staphylococcus aureus	-	betaine + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q9L4P8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
betaine aldehyde + NAD+ + H2O	-	Staphylococcus aureus	betaine + NADH + H+	-	?
betaine aldehyde + NADP+ + H2O	the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher	Staphylococcus aureus	betaine + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 60000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
BADH	-	Staphylococcus aureus
SACOL2628	-	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.56	-	NADP+	at pH 8.0 and 30°C	Staphylococcus aureus
6.18	-	NAD+	at pH 8.0 and 30°C	Staphylococcus aureus
11	-	Betaine aldehyde	at pH 8.0 and 30°C	Staphylococcus aureus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher	Staphylococcus aureus
NADP+	the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher	Staphylococcus aureus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.34	-	Betaine aldehyde	at pH 8.0 and 30°C	Staphylococcus aureus

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Release 2023.1 (January 2023)