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Literature summary for 1.2.1.8 extracted from

  • Oishi, H.; Ebina, M.
    Isolation of cDNA and enzymatic properties of betaine aldehyde dehydrogenase from Zoysia tenuifolia (2005), J. Plant Physiol., 162, 1077-1086.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pCR2.1-TOPO and pGEX-6p-2 vector for sequencing of the coding region and expression of the enzyme in Escherichia coli BL21, respectively Zoysia tenuifolia

Inhibitors

Inhibitors Comment Organism Structure
NaCl 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity Zoysia tenuifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
3-aminopropionaldehyde
-
Zoysia tenuifolia
0.049
-
4-Aminobutyraldehyde
-
Zoysia tenuifolia
0.291
-
Betaine aldehyde
-
Zoysia tenuifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55500
-
estimated by SDS-PAGE Zoysia tenuifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Zoysia tenuifolia
-
glycine betaine + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Zoysia tenuifolia Q6BD86 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BD88 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BD90 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BD91 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BD93 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BD95 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BD99 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BDA3 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
Zoysia tenuifolia Q6BDA4 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-

Purification (Commentary)

Purification (Comment) Organism
on GSTrap FF columns Zoysia tenuifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminopropionaldehyde + NAD+ + H2O
-
Zoysia tenuifolia 3-aminopropionic acid + NADH + H+
-
?
4-aminobutyraldehyde + NAD+ + H2O
-
Zoysia tenuifolia 4-aminobutyric acid + NADH + H+
-
?
betaine aldehyde + NAD+ + H2O
-
Zoysia tenuifolia glycine betaine + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
BADH
-
Zoysia tenuifolia
betaine aldehyde dehydrogenase
-
Zoysia tenuifolia
ZBD1
-
Zoysia tenuifolia
Zoysia betaine aldehyde dehydrogenase 1
-
Zoysia tenuifolia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
activity assay Zoysia tenuifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Zoysia tenuifolia
9.5
-
measurement of enzyme activity in dependence on pH, activity is strongly affected by the used buffer at the same pH Zoysia tenuifolia

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Zoysia tenuifolia