Cloned (Comment) | Organism |
---|---|
into the pCR2.1-TOPO and pGEX-6p-2 vector for sequencing of the coding region and expression of the enzyme in Escherichia coli BL21, respectively | Zoysia tenuifolia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity | Zoysia tenuifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
3-aminopropionaldehyde | - |
Zoysia tenuifolia | |
0.049 | - |
4-Aminobutyraldehyde | - |
Zoysia tenuifolia | |
0.291 | - |
Betaine aldehyde | - |
Zoysia tenuifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55500 | - |
estimated by SDS-PAGE | Zoysia tenuifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Zoysia tenuifolia | - |
glycine betaine + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoysia tenuifolia | Q6BD86 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BD88 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BD90 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BD91 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BD93 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BD95 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BD99 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BDA3 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Zoysia tenuifolia | Q6BDA4 | positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme | - |
Purification (Comment) | Organism |
---|---|
on GSTrap FF columns | Zoysia tenuifolia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropionaldehyde + NAD+ + H2O | - |
Zoysia tenuifolia | 3-aminopropionic acid + NADH + H+ | - |
? | |
4-aminobutyraldehyde + NAD+ + H2O | - |
Zoysia tenuifolia | 4-aminobutyric acid + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | - |
Zoysia tenuifolia | glycine betaine + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BADH | - |
Zoysia tenuifolia |
betaine aldehyde dehydrogenase | - |
Zoysia tenuifolia |
ZBD1 | - |
Zoysia tenuifolia |
Zoysia betaine aldehyde dehydrogenase 1 | - |
Zoysia tenuifolia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Zoysia tenuifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Zoysia tenuifolia |
9.5 | - |
measurement of enzyme activity in dependence on pH, activity is strongly affected by the used buffer at the same pH | Zoysia tenuifolia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zoysia tenuifolia |