Application | Comment | Organism |
---|---|---|
medicine | betaine aldehyde dehydrogenase is a potential target for antimicrobial agents against Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
wild type Pseudomonas aeruginosa betaine aldehyde dehydrogenase and the four mutants C353A, C377A, C439A and C286A | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C286A | cysteine 286 plays an important role in the maintenance ot the tetrameric structure | Pseudomonas aeruginosa |
C353A | cysteine 353 not essential for enzyme activity | Pseudomonas aeruginosa |
C377A | cysteine 377 not essential for enzyme activity | Pseudomonas aeruginosa |
C439A | cysteine 439 not essential for enzyme activity | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis[2-nitrobenzoic acid] | the effect of the thiol reagent DTNB on the native enzyme structure of the wild type enzyme and the mutants is examined | Pseudomonas aeruginosa | |
bis[diethylthiocarbamyl]disulfide | the effect of the thiol reagent disulfiram on the native enzyme structure of the wild type enzyme and the mutants is examined | Pseudomonas aeruginosa | |
S-methylmethanesulfonate | the effect of the thiol reagent MMTS on the native enzyme structure of the wild type enzyme and the mutants is examined | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD(P)+ + H2O | Pseudomonas aeruginosa | - |
glycine betaine + NAD(P)H + 2 H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified to homogeneity following a two-step procedure | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD(P)+ + H2O | - |
Pseudomonas aeruginosa | glycine betaine + NAD(P)H + 2 H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
betaine aldehyde dehydrogenase | - |
Pseudomonas aeruginosa |
betaine aldehyde:NAD(P)+ oxidoreductase | - |
Pseudomonas aeruginosa |
PaBADH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas aeruginosa |