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Literature summary for 1.2.1.70 extracted from
- Large scale production of biologically active Escherichia coli glutamyl-tRNA reductase from inclusion bodies (2003), Protein Expr. Purif., 31, 271-275.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression plasmid pBKCwt overexpression of a 6*His-tagged enzyme | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| inclusion body | - |
Escherichia coli | 16234 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamyl-tRNAGlu + NADPH + H+ | Escherichia coli | the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.47 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 6*His-tagged enzyme is stable for at least 6 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamyl-tRNAGlu + NADPH + H+ | - |
Escherichia coli | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? | |
| L-glutamyl-tRNAGlu + NADPH + H+ | the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes | Escherichia coli | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamate tRNA reductase | - |
Escherichia coli |
| glutamate-specific tRNA reductase | - |
Escherichia coli |
| glutamyl transfer RNA reductase | - |
Escherichia coli |
| glutamyl-tRNA reductase | - |
Escherichia coli |
| GluTR | - |
Escherichia coli |
| reductase, glutamyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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