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Literature summary for 1.2.1.70 extracted from
- Purification and partial characterization of barley glutamyl-tRNAGlu reductase,the enzyme that directs glutamate to chlorophyll biosynthesis (1994), Eur. J. Biochem., 225, 529-537.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 1 mM Cd(Ac)2, 92% inhibition | Hordeum vulgare |  |
| Co2+ | 10 mM CoCl2, 78% inhibition | Hordeum vulgare | |
| Cu2+ | 1 mM CuCl2, 84% inhibition | Hordeum vulgare | |
| Fe2+ | 5 mM FeSO4, 66% inhibition | Hordeum vulgare | |
| Fe3+ | 10 mM FeCl3, 80% inhibition | Hordeum vulgare | |
| glutamate 1-semialdehyde | 0.2 mM, 50% inhibition | Hordeum vulgare | |
| heme | 0.004 mM, 50% inhibition | Hordeum vulgare | |
| Zn2+ | 2.5 mM, ZnSO4, 94% inhibition | Hordeum vulgare | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | stroma of greening chloroplast | Hordeum vulgare | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Hordeum vulgare |
| 270000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamyl-tRNAGlu + NADPH + H+ | Hordeum vulgare | the enzyme directs glutamate to chlorophyll biosynthesis | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
cultivar Svalöf Bonus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamyl-tRNAGlu + NADPH + H+ | - |
Hordeum vulgare | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? | |
| L-glutamyl-tRNAGlu + NADPH + H+ | the enzyme directs glutamate to chlorophyll biosynthesis | Hordeum vulgare | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Hordeum vulgare |
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