Cloned (Comment) | Organism |
---|---|
gene vdh, phylogenetic tree | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
C292A | site-directed mutagensis, the mutant shows over 50% reduced activity compared to the wild-type enzyme. In presence of NADP+, the activity of N157A decreases to10% of the wild-type activity | Corynebacterium glutamicum |
E199A | site-directed mutagensis, the mutant shows over 50% reduced activity compared to the wild-type enzyme. In presence of NADP+ the activity of E199A decreases to 78% of the wild-type activity | Corynebacterium glutamicum |
E258A | site-directed mutagensis, the mutant shows over 50% reduced activity compared to the wild-type enzyme | Corynebacterium glutamicum |
K180A | site-directed mutagensis, the mutant shows over 50% reduced activity compared to the wild-type enzyme. In presence of NADP+, the activity of N157A decreases to10% of the wild-type activity | Corynebacterium glutamicum |
additional information | generation of a vdh deletion mutant, which partially loses its ability to grow on vanillin, indicating the presence of alternative VDH(s) in Corynebacterium glutamicum. When complemented with plasmid pXMJ19-vdhATCC13032, the growth ability of the mutant strain can be restored close to that of the wild type. The wild type, the DELTAvdhATCC13032 mutant and the complementary strain shows no difference when grown in p-cresol, cinnamyl aldehyde and syringaldehyde | Corynebacterium glutamicum |
N157A | site-directed mutagensis, the mutant shows over 50% reduced activity compared to the wild-type enzyme. In presence of NADP+, the activity of N157A decreases to10% of the wild-type activity | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49500 | - |
monomer, gel filtration | Corynebacterium glutamicum |
92300 | - |
dimer, gel filtration | Corynebacterium glutamicum |
159000 | - |
trimer, gel filtration | Corynebacterium glutamicum |
199200 | - |
tetramer, gel filtration | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillin + NAD+ + H2O | Corynebacterium glutamicum | - |
vanillate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NMB0 | - |
- |
Corynebacterium glutamicum ATCC 13032 | Q8NMB0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum | 3,4-dihydroxybenzoate + NADH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum ATCC 13032 | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum | benzoate + NADH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum ATCC 13032 | benzoate + NADH + 2 H+ | - |
? | |
cinnamaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum | cinnamate + NADH + 2 H+ | - |
? | |
cinnamaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum ATCC 13032 | cinnamate + NADH + 2 H+ | - |
? | |
additional information | product dentification by mass spectroscopic analysis | Corynebacterium glutamicum | ? | - |
? | |
additional information | product dentification by mass spectroscopic analysis | Corynebacterium glutamicum ATCC 13032 | ? | - |
? | |
o-phthaldialdehyde + NAD+ + H2O | - |
Corynebacterium glutamicum | ? + NADH + 2 H+ | - |
? | |
syringaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum | syringate + NADH + H+ | - |
? | |
syringaldehyde + NAD+ + H2O | - |
Corynebacterium glutamicum ATCC 13032 | syringate + NADH + H+ | - |
? | |
vanillin + NAD+ + H2O | - |
Corynebacterium glutamicum | vanillate + NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | VDHATCC13032 exists as tetramer, trimer and dimer | Corynebacterium glutamicum |
oligomer | x * 51000, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
VDH | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Corynebacterium glutamicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | activity range, profile overview | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Corynebacterium glutamicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9.5 | activity range, profile overview | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme can utilize NAD+ and NADP+ as coenzymes with similar efficiency and shows no obvious difference toward NAD+ and NADP+ | Corynebacterium glutamicum | |
NAD+ | - |
Corynebacterium glutamicum | |
NADP+ | - |
Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
malfunction | the vdh deletion mutant partially loses its ability to grow on vanillin, indicating the presence of alternative VDH(s) in Corynebacterium glutamicum. When complemented with plasmid pXMJ19-vdhATCC13032, the growth ability of the mutant strain can be restored close to that of the wild type. The wild type, the DELTAvdhATCC13032 mutant and the complementary strain shows no difference when grown in p-cresol, cinnamyl aldehyde and syringaldehyde | Corynebacterium glutamicum |
additional information | residues E258 and C292 are identified as the candidate conserved catalytic residues whereas N157, K180 and E199 are identified as the candidate cofactor interactive sites in VDHATCC13032, all residues are important for enzyme activity | Corynebacterium glutamicum |
physiological function | vanillin dehydrogenase is a crucial enzyme involved in the degradation of lignin-derived phenylpropanoids, such as vanillin, vanillate, caffeate, p-coumarate, and cinnamate | Corynebacterium glutamicum |