Cloned (Comment) | Organism |
---|---|
gene yfmT, DNA and amino acid sequence determination and analysis, recombinant overexpression of C-terminally HIs6-tagged enzyme in Escherchia coli strain JM109/pNG533.1 | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillin + NAD+ + H2O | Bacillus subtilis | - |
vanillate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 3NA | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | the cells of strain 3NA grow on ferulic acid, vanillin, vanillic acid, protocatechuic acid, and glucose as carbon sources, best on glucose, followed by protocatechuic acid, and low activity on the other carbon sources | Bacillus subtilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis | 3,4-dihydroxybenzoate + NADH + 2 H+ | - |
? | |
3-ethoxy-4-hydroxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis | 3-ethoxy-4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis | 3-hydroxy-4-methoxybenzoate + NADH + 2 H+ | - |
? | |
3-hydroxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis | 3-hydroxybenzoate + NADH + 2 H+ | - |
? | |
3-hydroxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis 3NA | 3-hydroxybenzoate + NADH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | - |
Bacillus subtilis 3NA | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Bacillus subtilis | benzoate + NADH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Bacillus subtilis 3NA | benzoate + NADH + 2 H+ | - |
? | |
additional information | 4-hydroxy-3-methoxycinnamaldehyde and cinnamaldehyde are poor substrates | Bacillus subtilis | ? | - |
? | |
additional information | 4-hydroxy-3-methoxycinnamaldehyde and cinnamaldehyde are poor substrates | Bacillus subtilis 3NA | ? | - |
? | |
salicylaldehyde + NAD+ + H2O | - |
Bacillus subtilis | salicylate + NADH + 2 H+ | - |
? | |
salicylaldehyde + NAD+ + H2O | - |
Bacillus subtilis 3NA | salicylate + NADH + 2 H+ | - |
? | |
vanillin + NAD+ + H2O | - |
Bacillus subtilis | vanillate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YfmT | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
inactivation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | in strain 3NA, ferulic acid is converted to a substance identified as 4-vinylguaiacol (2-methoxy-4-vinylphenol), and vanillin is converted to ca. 97% guaiacol and 3 % vanillyl alcohol, whereas vanillic acid is completely converted to guaiacol. Vanillin is converted to guaiacol, forming vanillic acid as an intermediate product | Bacillus subtilis |