Cloned (Comment) | Organism |
---|---|
cosmid library of SYK-6 strain transferred to Sphingomonas paucimobilis IAM12578. LigV expressed in Escherichia coli BL21(DE3) | Sphingomonas paucimobilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | insertional inactivation of ligV negatively affects growth on vanillin (only 11% of the vanillin dehydrogenase activity of the wild-type strain), whereas growth on syringaldehyde, veratraldehyde and coniferyl aldehyde is almost the same as the wild-type activity. Dehydrogenase activity towards benzaldehyde, p-hydroxybenzaldehyde, protocatechualdehyde and m-anisaldehyde is decreased to 27%, 35%, 14% and 54%, respectively of the wild-type activity. Mutant retains ability to grow on ferulate | Sphingomonas paucimobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50301 | - |
x * 50301, SDS-PAGE | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | A2PZP3 | - |
- |
Sphingomonas paucimobilis SYK-6 | A2PZP3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis SYK-6 | benzoate + NADH + H+ | - |
? | |
coniferyl aldehyde + NAD+ + H2O | activity toward coniferyl aldehyde is less than 5% of that toward vanillin | Sphingomonas paucimobilis | (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+ | - |
? | |
coniferyl aldehyde + NAD+ + H2O | activity toward coniferyl aldehyde is less than 5% of that toward vanillin | Sphingomonas paucimobilis SYK-6 | (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+ | - |
? | |
m-anisaldehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis | 3-methoxybenzoate + NADH + H+ | - |
? | |
m-anisaldehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis SYK-6 | 3-methoxybenzoate + NADH + H+ | - |
? | |
p-hydroxybenzaldehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis | 4-hydroxybenzoate + NADH + H+ | - |
? | |
p-hydroxybenzaldehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis SYK-6 | 4-hydroxybenzoate + NADH + H+ | - |
? | |
protocatechualdehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis | protocatechuate + NADH + H+ | - |
? | |
protocatechualdehyde + NAD+ + H2O | - |
Sphingomonas paucimobilis SYK-6 | protocatechuate + NADH + H+ | - |
? | |
syringaldehyde + NAD+ + H2O | activity toward syringaldehyde is less than 5% of that toward vanillin | Sphingomonas paucimobilis | syringate + NADH + H+ | - |
? | |
vanillin + NAD+ + H2O | best substrate | Sphingomonas paucimobilis | vanillate + NADH + H+ | - |
? | |
veratraldehyde + NAD+ + H2O | activity toward veratraldehyde is less than 5% of that toward vanillin | Sphingomonas paucimobilis | veratrate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50301, SDS-PAGE | Sphingomonas paucimobilis |
Synonyms | Comment | Organism |
---|---|---|
LigV | - |
Sphingomonas paucimobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | prefers NAD+ to NADP+ | Sphingomonas paucimobilis | |
NADP+ | - |
Sphingomonas paucimobilis |