Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.65 extracted from

  • Wang, Z.; Sun, Y.; Li, X.; Hu, H.; Zhang, C.
    A novel acetaldehyde dehydrogenase with salicylaldehyde dehydrogenase activity from Rhodococcus ruber strain OA1 (2017), Curr. Microbiol., 74, 1404-1410 .
    View publication on PubMed
Search for more literature for 1.2.1.65

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus ruber

Organism

Organism UniProt Comment Textmining
Rhodococcus ruber A0A291LTT3
-
-
Rhodococcus ruber OA1 A0A291LTT3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.82
-
 with formaldehyde as substrate, at pH 7.4 and 25°C Rhodococcus ruber
6.97
-
 with acetaldehyde as substrate, at pH 7.4 and 25°C Rhodococcus ruber
9.98
-
 with salicylaldehyde as substrate, at pH 7.4 and 25°C Rhodococcus ruber
10.42
-
 with benzaldehyde as substrate, at pH 7.4 and 25°C Rhodococcus ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Rhodococcus ruber acetate + NADH + 2 H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Rhodococcus ruber OA1 acetate + NADH + 2 H+
-
 ?
benzaldehyde + NAD+ + H2O highest substrate specificity for benzaldehyde Rhodococcus ruber benzoate + NADH + 2 H+
-
 ?
benzaldehyde + NAD+ + H2O highest substrate specificity for benzaldehyde Rhodococcus ruber OA1 benzoate + NADH + 2 H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Rhodococcus ruber formate + NADH + 2 H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Rhodococcus ruber OA1 formate + NADH + 2 H+
-
 ?
additional information the enzyme's salicylaldehyde dehydrogenase specific activity is higher than its aldehyde dehydrogenase specific activity Rhodococcus ruber ?
-
-
additional information the enzyme's salicylaldehyde dehydrogenase specific activity is higher than its aldehyde dehydrogenase specific activity Rhodococcus ruber OA1 ?
-
-
salicylaldehyde + NAD+ + H2O second highest substrate specificity for benzaldehyde Rhodococcus ruber salicylate + NADH + 2 H+
-
 ?
salicylaldehyde + NAD+ + H2O second highest substrate specificity for benzaldehyde Rhodococcus ruber OA1 salicylate + NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Rhodococcus ruber
? x * 30480, calculated from amino acid sequence Rhodococcus ruber

Synonyms

Synonyms Comment Organism
sALDH
-
 Rhodococcus ruber

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rhodococcus ruber