Application | Comment | Organism |
---|---|---|
additional information | two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occur in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria | Pseudomonas putida |
additional information | two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occurr in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) | Pseudomonas putida |
nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | inhibits NahF activity by 42%; inhibits NahV activity by 15% | Pseudomonas putida | |
Ba2+ | inhibits NahF activity by 62%; inhibits NahV activity by 52% | Pseudomonas putida | |
Ca2+ | inhibits NahF activity by 63%; inhibits NahV activity by 35% | Pseudomonas putida | |
Cs+ | inhibits NahF activity by 44%; inhibits NahV activity by 69% | Pseudomonas putida | |
Cu2+ | inhibits NahF activity by 70% | Pseudomonas putida | |
Li+ | inhibits NahF activity by 50%; inhibits NahV activity by 57% | Pseudomonas putida | |
Mg2+ | inhibits NahF activity by 34%; inhibits NahV activity by 36% | Pseudomonas putida | |
Ni2+ | inhibits NahF activity by 60%; inhibits NahV activity by 70% | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
NAD+ | - |
Pseudomonas putida | |
0.00109 | - |
NAD+ | - |
Pseudomonas putida | |
0.034 | - |
Salicylaldehyde | - |
Pseudomonas putida | |
0.146 | - |
Salicylaldehyde | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | enhances NahV activity by 63% activity | Pseudomonas putida | |
Fe2+ | enhances NahF activity by 40% activity | Pseudomonas putida | |
Fe2+ | enhances NahV activity by 152% | Pseudomonas putida | |
additional information | Zn2+ has no effect on NahF activity | Pseudomonas putida | |
Zn2+ | enhances NahV activity by 14% activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q6XUJ4 | - |
- |
Pseudomonas putida | Q6XUN9 | - |
- |
Pseudomonas putida ND6 | Q6XUJ4 | - |
- |
Pseudomonas putida ND6 | Q6XUN9 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged NahF purified by Ni-NTA chromatography | Pseudomonas putida |
His-tagged NahV purified by Ni-NTA chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-chlorosalicylaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 5-chlorosalicylate + NADH + H+ | - |
? | |
5-chlorosalicylaldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | 5-chlorosalicylate + NADH + H+ | - |
? | |
caprylic aldehyde + NAD+ + H2O | - |
Pseudomonas putida | caprylic acid + NADH + H+ | - |
? | |
caprylic aldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | caprylic acid + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Pseudomonas putida | formate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | formate + NADH + H+ | - |
? | |
glutaraldehyde + NAD+ + H2O | - |
Pseudomonas putida | glutarate + NADH + H+ | - |
? | |
glutaraldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | glutarate + NADH + H+ | - |
? | |
glyoxal + NAD+ + H2O | - |
Pseudomonas putida | glyoxylate + NADH + H+ | - |
? | |
m-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | m-nitrobenzoate + NADH + H+ | - |
? | |
o-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | o-methoxybenzoate + NADH + H+ | - |
? | |
o-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | o-methoxybenzoate + NADH + H+ | - |
? | |
o-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | o-nitrobenzoate + NADH + H+ | - |
? | |
salicylaldehyde + NAD+ + H2O | best substrate | Pseudomonas putida | salicylate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NahF | - |
Pseudomonas putida |
NahV | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
72.8% activity is retained for NahF after a 30 min exposure | Pseudomonas putida |
50 | - |
NahV looses 88.8% activity after a 30 min exposure | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas putida | - |
- |
5.08 |
Pseudomonas putida | - |
- |
5.63 |