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Literature summary for 1.2.1.64 extracted from

  • Mukherjee, C.; Samanta, T.; Mitra, A.
    Redirection of metabolite biosynthesis from hydroxybenzoates to volatile terpenoids in green hairy roots of Daucus carota (2016), Planta, 243, 305-320 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzaldehyde + NAD+ + H2O Daucus carota
-
4-hydroxybenzoate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Daucus carota
-
var. early nantes
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root green hairy root cultures. Hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots and 15fold less compared to normal hairy roots. Volatile analysis of 14-day-old green hairy roots reveals higher monoterpene and sesquiterpene contents than found in normal hairy roots, methyl salicylate content is higher in normal hairy roots than in green ones, overview Daucus carota
-
root
-
Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000024
-
green hairy roots, pH 7.5, 35°C Daucus carota
0.00036
-
normal hairy roots, pH 7.5, 35°C Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzaldehyde + NAD+ + H2O
-
Daucus carota 4-hydroxybenzoate + NADH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
HBD
-
Daucus carota
p-hydroxybenzaldehyde dehydrogenase
-
Daucus carota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Daucus carota

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Daucus carota

Expression

Organism Comment Expression
Daucus carota hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots down

General Information

General Information Comment Organism
malfunction hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots. Volatile analysis of 14-day-old green hairy roots reveals higher monoterpene and sesquiterpene contents than found in normal hairy roots, methyl salicylate content is higher in normal hairy roots than in green ones, metabolic phenotype, overview Daucus carota
metabolism the enzyme is involved in the phenylpropanoid/hydroxybenzoate pathway in the plant, pathway overview Daucus carota
physiological function plant hydroxybenzoates are common mediators of plant responses of biotic and abiotic stress Daucus carota