BRENDA - Enzyme Database show
show all sequences of 1.2.1.59

Gluconeogenesis from pyruvate in the hyperthermophilic archaeon Pyrococcus furiosus involvement of reactions of the Embden-Meyerhof pathway

Schäfer, T.; Schönheit, P.; Arch. Microbiol. 159, 354-363 (1993)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
NADPH
50°C, pH not specified in the publication
Pyrococcus furiosus
0.05
-
NADP+
50°C, pH not specified in the publication
Pyrococcus furiosus
1.4
-
NADH
50°C, pH not specified in the publication
Pyrococcus furiosus
1.6
-
NAD+
50°C, pH not specified in the publication
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
P61879
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.009
-
50°C, pH not specified in the publication, cofactor: NAD+, in presence of arsenate
Pyrococcus furiosus
0.019
-
50°C, pH not specified in the publication, cofactor: NADP+, in presence of arsenate
Pyrococcus furiosus
0.035
-
50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH
Pyrococcus furiosus
0.06
-
50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
744178
Pyrococcus furiosus
3-phospho-D-glyceroyl phosphate + NADH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
NADP+ is the preferred cofactor
744178
Pyrococcus furiosus
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Pyrococcus furiosus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pyrococcus furiosus
NADP+
NADP+ is the preferred cofactor
Pyrococcus furiosus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pyrococcus furiosus
NADP+
NADP+ is the preferred cofactor
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
NADPH
50°C, pH not specified in the publication
Pyrococcus furiosus
0.05
-
NADP+
50°C, pH not specified in the publication
Pyrococcus furiosus
1.4
-
NADH
50°C, pH not specified in the publication
Pyrococcus furiosus
1.6
-
NAD+
50°C, pH not specified in the publication
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.009
-
50°C, pH not specified in the publication, cofactor: NAD+, in presence of arsenate
Pyrococcus furiosus
0.019
-
50°C, pH not specified in the publication, cofactor: NADP+, in presence of arsenate
Pyrococcus furiosus
0.035
-
50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH
Pyrococcus furiosus
0.06
-
50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
744178
Pyrococcus furiosus
3-phospho-D-glyceroyl phosphate + NADH + H+
-
-
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
NADP+ is the preferred cofactor
744178
Pyrococcus furiosus
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Pyrococcus furiosus
Expression
Organism
Commentary
Expression
Pyrococcus furiosus
induced 10fold under growth conditions which require gluconeogenesis
up
Expression (protein specific)
Organism
Commentary
Expression
Pyrococcus furiosus
induced 10fold under growth conditions which require gluconeogenesis
up
Other publictions for EC 1.2.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Ayres
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1
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1
1
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6
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9
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1
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10
10
743026
Jiang
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725013
Ito
Comparative analysis of two gl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
FEBS Lett.
586
3097-3103
2012
1
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1
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7
7
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723857
Tourigny
Expression, purification, crys ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Acta Crystallogr. Sect. F
67
72-75
2011
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1
1
1
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15
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725853
Kim
Alteration of reducing powers ...
Bacillus subtilis 168, Bacillus subtilis
Lett. Appl. Microbiol.
52
433-440
2011
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1
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5
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726605
Malay
Structure of glyceraldehyde-3- ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Acta Crystallogr. Sect. F
65
1227-1233
2009
-
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1
1
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4
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656414
Marri
Co-ordinated gene expression o ...
Arabidopsis thaliana
J. Exp. Bot.
56
73-80
2005
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1
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1
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11538
Valverde
Functional complementation of ...
Anabaena sp., Calothrix sp., Fischerella sp., Fischerella sp. UTEX 1829, Methanobacterium bryantii, Methanobacterium formicicum, Methanothermus fervidus, Nostoc sp., Pseudanabaena sp., Pseudanabaena sp. PCC6903, Stanieria sp., Synechococcus sp., Synechocystis sp.
J. Bacteriol.
179
4513-4522
1997
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1
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4
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2
1
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17
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1
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28
2
1
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1
1
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1
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4
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1
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1
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28
2
1
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1
1
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11542
Serrano
-
Identification of two differen ...
Cyanophora paradoxa
Arch. Microbiol.
162
14-19
1994
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2
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2
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1
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744178
Schäfer
-
Gluconeogenesis from pyruvate ...
Pyrococcus furiosus
Arch. Microbiol.
159
354-363
1993
-
-
-
-
-
-
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4
-
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1
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4
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2
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1
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2
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2
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4
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4
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2
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1
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1
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1
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11537
Zwickl
Glyceraldehyde-3-phosphate deh ...
Pyrococcus woesei
J. Bacteriol.
172
4329-4338
1990
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1
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1
1
2
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7
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11539
Fabry
Purification and characterizat ...
Methanothermus fervidus
Eur. J. Biochem.
165
147-155
1987
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1
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2
1
4
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2
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1
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1
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1
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11541
Papen
-
Properties of the glyceraldehy ...
Anabaena cylindrica, Anabaena sp., Anabaena sp. 7119, Trichormus variabilis
FEMS Microbiol. Lett.
36
201-206
1986
9
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11540
Krusteva
-
Allosteric regulation of NAD(N ...
Auxenochlorella pyrenoidosa
FEBS Lett.
171
137-140
1984
6
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