Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta DE3 cells | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzyme C150S, sitting drop vapor diffusion method, using 25% (w/v) PEG 1K, 175 mM sodium/potassium tartrate and 100 mM sodium acetate pH 4.5 | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
C150S | the mutant is unable to turn over D-glyceraldehyde 3-phosphate in the presence of either NAD+ or NADP+ | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Campylobacter jejuni | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Campylobacter jejuni NCTC 11168 | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | Campylobacter jejuni | - |
3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | Campylobacter jejuni NCTC 11168 | - |
3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Campylobacter jejuni NCTC 11168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap nickel Sepharose column chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Campylobacter jejuni | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Campylobacter jejuni NCTC 11168 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Campylobacter jejuni | 3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Campylobacter jejuni NCTC 11168 | 3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cgap | - |
Campylobacter jejuni |
GAPDH | - |
Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme can utilize either NADP+ or NAD+ as coenzymes | Campylobacter jejuni | |
NADP+ | the enzyme can utilize either NADP+ or NAD+ as coenzymes | Campylobacter jejuni |