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Literature summary for 1.2.1.59 extracted from

  • Valverde, F.; Losada, M.; Serrano, A.
    Functional complementation of an Escherichia coli gap mutant supports an amphibolic role for NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechocystis sp. strain PCC 6803 (1997), J. Bacteriol., 179, 4513-4522.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.59

Cloned(Commentary)

Cloned (Comment) Organism
cloned by functional complementation of an Escherichia coli gap mutant Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH
-
 Synechocystis sp.
0.01
-
 NADH
-
 Synechocystis sp.
0.03
-
 NAD(P)+
-
 Synechocystis sp.
0.27
-
 D-glyceraldehyde 3-phosphate with NAD+ or NADP+ as cosubstrate Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 4 * 38000, SDS-PAGE Synechocystis sp.
160000
-
 FPLC gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Synechocystis sp. possible amphibolic role: anabolic in photosynthetic carbon assimilation and catabolic in carbohydrate degradative pathways ?
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
 strain PCC 7120, strain ATCC 29413
-
Calothrix sp.
-
 strain PCC 7601
-
Fischerella sp.
-
-
-
Fischerella sp. UTEX 1829
-
-
-
Methanobacterium bryantii
-
-
-
Methanobacterium formicicum
-
-
-
Methanothermus fervidus
-
-
-
Nostoc sp.
-
 strain PCC 7107
-
Pseudanabaena sp.
-
-
-
Pseudanabaena sp. PCC6903
-
-
-
Stanieria sp.
-
 strain PCC 7437
-
Synechococcus sp.
-
 strain PCC 7942
-
Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH + H+
-
 Synechocystis sp. D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Synechococcus sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Anabaena sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Methanobacterium bryantii 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Methanobacterium formicicum 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Stanieria sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Pseudanabaena sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Nostoc sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Calothrix sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Fischerella sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+ r Methanothermus fervidus 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+ r Synechocystis sp. 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Fischerella sp. UTEX 1829 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Pseudanabaena sp. PCC6903 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Synechococcus sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Anabaena sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Methanobacterium bryantii 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Methanobacterium formicicum 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Stanieria sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Pseudanabaena sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Nostoc sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Calothrix sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Fischerella sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+ r Methanothermus fervidus 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+ r Synechocystis sp. 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Fischerella sp. UTEX 1829 1,3-diphosphoglycerate + NADPH
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Pseudanabaena sp. PCC6903 1,3-diphosphoglycerate + NADPH
-
 ?
additional information high hydrolase activity with a variety of organic p-nitrophenyl esters Synechocystis sp. ?
-
 ?
additional information possible amphibolic role: anabolic in photosynthetic carbon assimilation and catabolic in carbohydrate degradative pathways Synechocystis sp. ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Methanothermus fervidus
tetramer 4 * 38000, SDS-PAGE Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Synechocystis sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 considerable activity in the range of pH 7 to pH 10 Synechocystis sp.