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Literature summary for 1.2.1.51 extracted from
- Purification and characterization of pyruvate:NADP+ oxidoreductase in Euglena gracilis (1987), J. Biol. Chem., 262, 9130-9135.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not arsenite | Euglena gracilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
CoA | - |
Euglena gracilis |  |
| 0.03 | - |
NADP+ | - |
Euglena gracilis | |
| 0.21 | - |
pyruvate | - |
Euglena gracilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 4 mol per mol subunit | Euglena gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 166000 | - |
2 * 166000, SDS-PAGE | Euglena gracilis |
| 309000 | - |
gel filtration | Euglena gracilis |
| 309000 | - |
I. methyl viologen-pyruvate dehydrogenase fragment with iron-sulfur cluster | Euglena gracilis |
| 309000 | - |
two covalently linked domains | Euglena gracilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + NADP+ | Euglena gracilis | - |
acetyl-CoA + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| O2 leads to rapid inactivation | Euglena gracilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.93 | - |
3-hydroxypyruvate | Euglena gracilis |
| 2.36 | - |
2-oxobutyrate | Euglena gracilis |
| 2.6 | - |
oxaloacetate | Euglena gracilis |
| 14.3 | - |
pyruvate | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxobutyrate + CoA + NADP+ | - |
Euglena gracilis | propionyl-CoA + CO2 + NADPH | - |
? | |
| 3-hydroxypyruvate + CoA + NADP+ | - |
Euglena gracilis | hydoxyacetyl-CoA + CO2 + NADPH | - |
? | |
| oxaloacetate + CoA + NADP+ | - |
Euglena gracilis | ? + CO2 + NADPH | - |
? | |
| pyruvate + CoA + NADP+ | - |
Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
| pyruvate + CoA + NADP+ | NADP+ can be substituted by methyl viologen | Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
| pyruvate + CoA + NADP+ | NADP+ can be substituted by benzyl viologen | Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
| pyruvate + CoA + NADP+ | reverse reaction 2% of forward reaction | Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 166000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Euglena gracilis | |
| NADP+ | not NAD+ | Euglena gracilis | |
| thiamine diphosphate | - |
Euglena gracilis | |
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