Cloned (Comment) | Organism |
---|---|
cloning of CpFAS1-R domain in a pMAL-c2x vector with maltose binding protein-fusion protein and expression in Escherichia coli | Cryptosporidium parvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
recombinant CpFAS1-R domain as maltose binding protein fusion protein, SDS-PAGE | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin-based chromatography purification | Cryptosporidium parvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain acyl-CoA + NADPH + H+ | - |
Cryptosporidium parvum | a long-chain aldehyde + CoA + NADP+ | - |
? | |
additional information | enzyme only utilizes very long chain fatty acyl-CoAs as substrates, with activity on C26 > C24 > C22 > C20, but no activity on C18 and C16, enzyme is capable of using NADPH and NADH as electron donors, but prefers NADPH to NADH | Cryptosporidium parvum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpFAS1-R | - |
Cryptosporidium parvum |
General Information | Comment | Organism |
---|---|---|
metabolism | CpFAS1-R enzyme is part of the CpFAS1 multifunctional Type I fatty acid synthase complex with at least 21 enzymatic domains, complex is predicted to function as a fatty acyl elongase preferring long chain fatty acids as substrates | Cryptosporidium parvum |